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Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
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| Tese | 3.2 MB | Adobe PDF |
Orientador(es)
Resumo(s)
Botrytis cinerea [teleomorph: Botryotinia fuckeliana] is a heterothallic filamentous
plant pathogenic Ascomycete with over two hundred different hosts, and its control at
the crop level is still very difficult to achieve. Sexual reproduction as an active part in
the fungus dispersal and variability has been underestimated until recent years.
Research leading to more insight into the MAT locus and the fundamental principles of
the mating process can prompt new strategies for B. cinerea control. During the
B. cinerea infection process, phytotoxic proteins such as NEP1 are produced. Their
function is yet to be unraveled, but it was postulated that they might play a role in
speciation in the genus Botrytis.
This report describes a mating type screening of a population of 80 field isolates of
B. cinerea. Findings indicate a typical 1:1 Mendelian distribution of the MAT loci, and
one strain with both MAT genes is reported. A scan of the MAT locus suggests the
absence of the MAT1-1 locus in dual mating strains. Furthermore, Southern blotting
demonstrates for the first time the possible presence of a complete MAT1-1 locus in a
dual mating strain, instead of the predicted incomplete MAT1-1 locus (dMAT1).
Besides the MAT loci, genomic sequences of the BcNEP1 gene in 16 different B. cinerea
strains are analyzed, and results demonstrate the existence of three haplotypes and
two alleles. Purifying selection is accounted for evolutionary changes within the gene.Finally, four uORF independent knockout mutants were created. This predicted gene is
located within the MAT1-1 locus, but its function is yet to be described.
Descrição
Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
Palavras-chave
Botrytis cinerea genes MAT proteinas NEP silenciamento genético MAT genes NEP proteins gene knockout