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Low Temperature Dissolution of Yeast Chitin-Glucan Complex and Characterization of the Regenerated Polymer

2020Journal article Open access
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dc.contributor.authorAraújo, Diana
dc.contributor.authorDelgado Alves, Vitor
dc.contributor.authorMarques, Ana C.
dc.contributor.authorFortunato, Elvira
dc.contributor.authorReis, Maria A.M.
dc.contributor.authorFreitas, Filomena
dc.date.accessioned2021-09-29T11:53:27Z
dc.date.available2021-09-29T11:53:27Z
dc.date.issued2020
dc.description.abstractChitin-glucan complex (CGC) is a copolymer composed of chitin and glucan moieties extracted from the cell-walls of several yeasts and fungi. Despite its proven valuable properties, that include antibacterial, antioxidant and anticancer activity, the utilization of CGC in many applications is hindered by its insolubility in water and most solvents. In this study, NaOH/urea solvent systems were used for the first time for solubilization of CGC extracted from the yeast Komagataella pastoris. Di erent NaOH/urea ratios (6:8, 8:4 and 11:4 (w/w), respectively) were used to obtain aqueous solutions using a freeze/thaw procedure. There was an overall solubilization of 63–68%, with the highest solubilization rate obtained for the highest tested urea concentration (8 wt%). The regenerated polymer, obtained by dialysis of the alkali solutions followed by lyophilization, formed porous macrostructures characterized by a chemical composition similar to that of the starting co-polymer, although the acetylation degree decreased from 61.3% to 33.9–50.6%, indicating that chitin was converted into chitosan, yielding chitosan-glucan complex (ChGC). Consistent with this, there was a reduction of the crystallinity index and thermal degradation temperature. Given these results, this study reports a simple and green procedure to solubilize CGC and obtain aqueous ChGC solutions that can be processed as novel biomaterialspt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBioengineering 2020, 7, 28pt_PT
dc.identifier.doi10.3390/bioengineering7010028pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/22098
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUID/AGR/04129/2020pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectchitin-glucan complex (CGC)pt_PT
dc.subjectchitosan-glucan complex (ChGC)pt_PT
dc.subjectNaOH/urea solvent systemspt_PT
dc.subjectdissolutionpt_PT
dc.subjectstructural analysispt_PT
dc.subjectthermal propertiespt_PT
dc.titleLow Temperature Dissolution of Yeast Chitin-Glucan Complex and Characterization of the Regenerated Polymerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157549/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/154767/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PTDC%2FEME-MFE%2F71960%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Orçamento de Funcionamento%2FPOSC/POSC%2FEEA-SRI%2F61911%2F2004/PT
oaire.citation.titleBioengineeringpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStreamOrçamento de Funcionamento/POSC
person.familyNameDelgado Alves
person.givenNameVitor
person.identifier1916677
person.identifier.ciencia-id2E1E-6918-4DB3
person.identifier.orcid0000-0002-4117-5582
person.identifier.scopus-author-id24536716000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery8d1a8489-8789-4569-89e2-0bade23dd945
relation.isProjectOfPublicationb69df505-c1cb-42d5-b901-59998ffbc4da
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