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Influência da texturização de superfícies de zircónia por laser Nd:YAG à nano e micro escala no comportamento celular de fibroblastos
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Objetivos: Avaliar o comportamento celular de fibroblastos (HGF-hTERT) em superfícies de Zircónia texturizadas por laser Nd:YAG comparativamente com superfícies convencionalmente texturizadas por jateamento e ataque ácido.
Métodos: Prepararam-se discos de Zircónia e distribuíram-se aleatoriamente pelos grupos em estudo (N=12): sem tratamento (Grupo A); jateamento e ataque ácido (Grupo B); texturização por laser Nd:YAG espaçamento de 10 µm/ 10 passagens (Grupo C); texturização por laser Nd:YAG espaçamento de 20 µm/1 passagem (Grupo D). Cultivaram-se HGF-hTERT nestas superfícies. A viabilidade celular foi avaliada aos 1, 3 e 7 dias usando um método à base de resazurina. A morfologia e adesão celulares foram analisadas por microscopia de fluorescência e microscopia eletrónica de varrimento (FEG-SEM) aos 1 e 3 dias. A produção de interleucina 6 (IL-6) e interleucina 1β (IL-1β) foi medida aos 1 e 3 dias por ensaios enzimáticos de imunoabsorção (ELISA). Os resultados foram apresentados como média ± desvio padrão. Realizaram-se comparações entre grupos por ANOVA unidirecional e de medidas repetidas (teste post-hoc de Tukey) usando um software de estatística. A significância foi definida como p<0,05.
Resultados: A viabilidade celular aumentou ao longo do tempo para todos os grupos. Aos 1 e
7 dias verificaram-se valores de viabilidade significativamente superiores no Grupo C comparativamente ao Grupo B (p<0,05). As imagens de microscopia revelaram adesão em ambos os tempos, com morfologia normal e prolongamentos celulares sobre as superfícies de todos os discos. Observou-se um maior número de células aderidas nos Grupos C e D do que nos outros grupos, em ambos os tempos. Não foram observadas diferenças na produção de IL- 6 e IL-1β entre os grupos em estudo (p>0,05).
Conclusões: As superfícies implantares de Zircónia texturizadas por laser parecem beneficiar a resposta de fibroblastos comparativamente a superfícies tratadas com jateamento e ataque ácido. Mais estudos devem ser realizados para validar esta técnica de texturização.
Objectives: To evaluate fibroblast behavior (HGF-hTERT) in Zirconia Nd:YAG laser textured surfaces compared to sandblasted and acid etched surfaces. Methods: Zirconia discs were prepared and randomly distributed among the study groups (N = 12): without treatment (Group A); sandblasting and acid etching (Group B); texturing by Nd:YAG laser spacing of 10 µm / 10 passages (Group C); texturing by Nd:YAG laser spacing of 20 µm / 1 passage (Group D). HGF-hTERT were grown on these surfaces. Cell viability was assessed at 1, 3 and 7 days using a resazurin-based method. Cell morphology and adhesion were analyzed by fluorescence microscopy and scanning electron microscopy (FEG-SEM) at 1 and 3 days. The production of interleukin 6 (IL-6) and interleukin 1β (IL-1β) was measured at 1 and 3 days by enzyme immunosorbent assays (ELISA). The results were presented as mean ± standard deviation. Comparisons between groups were performed by unidirectional and a repeated measures ANOVA (Tukey's post-hoc test) using statistical software. Significance was defined as p <0.05. Results: Cell viability increased over time for all groups. At 1 and 7 days, viability values were significantly higher in Group C compared to Group B (p <0.05). Microscopy images revealed adhesion at both times, with normal morphology and cellular extensions on the surfaces of all discs. A greater number of adhered cells was observed in Groups C and D than in the other groups, at both times. There were no differences in the production of IL-6 and IL-1β between the study groups (p> 0.05). Conclusions: Laser textured Zirconia implant surfaces appear to benefit the fibroblast response compared to surfaces treated with sandblasting and acid etching. Further studies must be carried out to validate this texturing technique.
Objectives: To evaluate fibroblast behavior (HGF-hTERT) in Zirconia Nd:YAG laser textured surfaces compared to sandblasted and acid etched surfaces. Methods: Zirconia discs were prepared and randomly distributed among the study groups (N = 12): without treatment (Group A); sandblasting and acid etching (Group B); texturing by Nd:YAG laser spacing of 10 µm / 10 passages (Group C); texturing by Nd:YAG laser spacing of 20 µm / 1 passage (Group D). HGF-hTERT were grown on these surfaces. Cell viability was assessed at 1, 3 and 7 days using a resazurin-based method. Cell morphology and adhesion were analyzed by fluorescence microscopy and scanning electron microscopy (FEG-SEM) at 1 and 3 days. The production of interleukin 6 (IL-6) and interleukin 1β (IL-1β) was measured at 1 and 3 days by enzyme immunosorbent assays (ELISA). The results were presented as mean ± standard deviation. Comparisons between groups were performed by unidirectional and a repeated measures ANOVA (Tukey's post-hoc test) using statistical software. Significance was defined as p <0.05. Results: Cell viability increased over time for all groups. At 1 and 7 days, viability values were significantly higher in Group C compared to Group B (p <0.05). Microscopy images revealed adhesion at both times, with normal morphology and cellular extensions on the surfaces of all discs. A greater number of adhered cells was observed in Groups C and D than in the other groups, at both times. There were no differences in the production of IL-6 and IL-1β between the study groups (p> 0.05). Conclusions: Laser textured Zirconia implant surfaces appear to benefit the fibroblast response compared to surfaces treated with sandblasting and acid etching. Further studies must be carried out to validate this texturing technique.
Descrição
Tese de mestrado, Medicina Dentária, Universidade de Lisboa, Faculdade de Medicina Dentária, 2020
Palavras-chave
Saúde Oral Teses de mestrado - 2020