Publicação
Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance
| dc.contributor.author | Figueira, Tiago N. | |
| dc.contributor.author | Freire, João M. | |
| dc.contributor.author | Santos, Catarina Cunha | |
| dc.contributor.author | Heras, Montserrat | |
| dc.contributor.author | Gonçalves, João | |
| dc.contributor.author | Moscona, Anne | |
| dc.contributor.author | Porotto, Matteo | |
| dc.contributor.author | Veiga, Ana Salomé | |
| dc.contributor.author | Castanho, Miguel A. R. B. | |
| dc.date.accessioned | 2018-04-02T14:54:38Z | |
| dc.date.available | 2018-04-02T14:54:38Z | |
| dc.date.issued | 2017 | |
| dc.description | © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.description.abstract | Understanding the interplay between molecules and lipid membranes is fundamental when studying cellular and biotechnological phenomena. Partition between aqueous media and lipid membranes is key to the mechanism of action of many biomolecules and drugs. Quantifying membrane partition, through adequate and robust parameters, is thus essential. Surface Plasmon Resonance (SPR) is a powerful technique for studying 1:1 stoichiometric interactions but has limited application to lipid membrane partition data. We have developed and applied a novel mathematical model for SPR data treatment that enables determination of kinetic and equilibrium partition constants. The method uses two complementary fitting models for association and dissociation sensorgram data. The SPR partition data obtained for the antibody fragment F63, the HIV fusion inhibitor enfuvirtide, and the endogenous drug kyotorphin towards POPC membranes were compared against data from independent techniques. The comprehensive kinetic and partition models were applied to the membrane interaction data of HRC4, a measles virus entry inhibitor peptide, revealing its increased affinity for, and retention in, cholesterol-rich membranes. Overall, our work extends the application of SPR beyond the realm of 1:1 stoichiometric ligand-receptor binding into a new and immense field of applications: the interaction of solutes such as biomolecules and drugs with lipids. | pt_PT |
| dc.description.sponsorship | Project HIVERA/0002/2013 is acknowledged. T.N.F. acknowledges fellowship SFRH/BD/52383/2013 and A.S.V. acknowledges funding under the FCT Investigator Programme (IF/00803/2012) from Fundacao para a Ciencia e Tecnologia (FCT), Portugal. The work was partially supported by grants from NIAID AI109050 to M.P. and AI114736 to A.M. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Sci. Rep. 7, 45647 | pt_PT |
| dc.identifier.doi | 10.1038/srep45647 | pt_PT |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | http://hdl.handle.net/10451/32541 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Nature Publishing Group | pt_PT |
| dc.relation | SFRH/BD/52383/2013 | pt_PT |
| dc.relation | IF/00803/2012 | pt_PT |
| dc.relation.publisherversion | https://www.nature.com/srep/ | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | 45647 | pt_PT |
| oaire.citation.title | Scientific Reports | pt_PT |
| oaire.citation.volume | 7 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |