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Higher membrane fluidity mediates the increased subcutaneous fatty acid content in pigs fed reduced protein diets

2017Journal article Restricted access
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dc.contributor.authorLopes, P. A.
dc.contributor.authorMartins, A. P.
dc.contributor.authorMartins, S. V.
dc.contributor.authorMadeira, M. S.
dc.contributor.authorSantos, N. C.
dc.contributor.authorMoura, T. F.
dc.contributor.authorPrates, J. A. M.
dc.contributor.authorSoveral, G.
dc.date.accessioned2018-06-05T13:55:07Z
dc.date.available2018-06-05T13:55:07Z
dc.date.issued2017
dc.description© The Animal Consortium 2016pt_PT
dc.description.abstractThe production of pork with moderate amounts of intramuscular fat (IMF) without an increase in subcutaneous fat is highly desirable for the meat industry. Several studies indicate that dietary protein reduction during the growing-finishing period of pigs enhances IMF content, but its consequence on carcass fat deposition is still contradictory. In this study, we hypothesized that the effects of reduced protein diets (RPD), corrected or not with the limiting amino acid lysine, on subcutaneous fat deposition from pigs with distinct genotypes are mediated by adipose membranes biophysical properties. In total, 36 crossbred (Large White×Landrace×Pietrain - a lean genotype) and purebred (Alentejana breed - a fatty genotype) male pigs were randomly assigned to the control group, the RPD group or the reduced protein diet equilibrated for lysine (RPDL) group, allowing a 2×3 factorial arrangement (n=6). Backfat thickness and total fatty acid content were higher in Alentejana relative to crossbred pigs. Although dietary treatments did not change backfat thickness, RPD and RPDL increased total fatty acids content of subcutaneous fat. In order to understand this effect, adipose tissue membranes isolated from pig's subcutaneous fat were assayed for glycerol permeability and fluidity, using 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-(trimethylamino)-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) probes. The glycerol transport across adipose membranes was not mediated by aquaglyceroporins and remained unchanged across dietary groups. Regardless of lysine correction, RPD increased membrane fluidity at the hydrocarbon region (lower DPH fluorescence anisotropy) in both genotypes of pigs. This result was associated with a lower ratio between oleic acid and linoleic acid on membrane's fatty acid composition. Adipose membrane's cholesterol content was independent from genotype and diet. Taken together, the present study shows that dietary protein reduction is successful in maintaining backfat thickness, although a negative side effect was observed on total fatty acids in subcutaneous fat, which may be due to changes in the fluidity of adipose membranes.pt_PT
dc.description.sponsorshipFinancial support from Fundação para a Ciência e a Tecnologia – Ministério da Educação e Ciência (FCT-MEC, Portugal) grant (PTDC/CVT/2008/99210), CIISA project (UID/CVT/2013/00276) and individual fellowships to A. P. M. (SFRH/BD/2009/65046), S. V. M. (SFRH/BPD/2009/63019) and M. S. M. (SFRH/BD/2008/48240) are acknowledged. P. A. L. is a researcher from the FCT-MEC program ‘Ciência 2008’ and Incentivo 2014 project (AGR/UI0276/2014).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAnimal (2017), 11:4, pp 713–719pt_PT
dc.identifier.doi10.1017/S1751731116001968pt_PT
dc.identifier.issn1751-7311
dc.identifier.urihttp://hdl.handle.net/10451/33754
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCambridge University Presspt_PT
dc.relation.publisherversionhttps://www.cambridge.org/core/journals/animalpt_PT
dc.subjectGlycerol permeabilitypt_PT
dc.subjectMembrane fluiditypt_PT
dc.subjectPigspt_PT
dc.subjectReduced protein dietspt_PT
dc.subjectSubcutaneous fatpt_PT
dc.titleHigher membrane fluidity mediates the increased subcutaneous fatty acid content in pigs fed reduced protein dietspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT%2F099210%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCVT%2F00276%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F65046%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63019%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F48240%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/Incentivo%2FAGR%2FUI0276%2F2014/PT
oaire.citation.endPage719pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage713pt_PT
oaire.citation.titleanimalpt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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