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Influence of dietary Spirulina inclusion and lysozyme supplementation on the longissimus lumborum muscle proteome of newly weaned piglets

2021Journal article Open access
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dc.contributor.authorRibeiro, David M.
dc.contributor.authorMartins, Cátia F.
dc.contributor.authorKules, Josipa
dc.contributor.authorHorvatic, Anita
dc.contributor.authorGuillemin, Nicolas
dc.contributor.authorFreire, João
dc.contributor.authorEckersall, P. David
dc.contributor.authorAlmeida, André M.
dc.contributor.authorPrates, José A.M.
dc.date.accessioned2021-09-16T14:58:10Z
dc.date.available2021-09-16T14:58:10Z
dc.date.issued2021
dc.description.abstractArthrospira platensis (Spirulina) is a microalga with a high content of crude protein. Its recalcitrant cell wall limits the accessibility of the animal endogenous enzymes to its intracellular nutrients. Enzymatic supplementation aiming to degrade cell walls could benefit microalgae digestibility. The objective of this study was to evaluate the impact of dietary Spirulina and lysozyme supplementation over the muscle proteome of piglets during the post-weaning stage. Thirty piglets were randomly distributed among three diets: control (no microalga), SP (10% Spirulina) and SP+L (10% Spirulina + 0.01% lysozyme). After 4 weeks, they were sacrificed and samples of the longissimus lumborum muscle were taken. The muscle proteome was analysed using a Tandem Mass Tag (TMT)-based quantitative approach. A total of 832 proteins were identified. Three comparisons were computed: SP vs Ctrl, SP+L vs Ctrl and SP+L vs SP. They had ten, four and twelve differentially abundant proteins. Glycogen metabolism and nutrient reserves utilization are seemingly increased in the SP piglets. Structural muscle protein synthesis increased, causing higher energy requirements in SP+L piglets. Our results demonstrate the usefulness of proteomics to disclose the effect of dietary microalgae, whilst unveiling putative mechanisms derived from lysozyme supplementation. Data are available via ProteomeXchange with identifier PXD024083pt_PT
dc.description.versioninfo:eu-repo/semantics/submittedVersionpt_PT
dc.identifier.citationRibeiro DM, Martins CF, Kuleš J, Horvatić A, Guillemin N, Freire JPB, Eckersall PD, Almeida AM, Prates JAM (2021). Influence of dietary Spirulina inclusion and lysozyme supplementation on the longissimus lumborum muscle proteome of newly weaned piglets. J Proteomics. 2021 Jul 30;244:104274pt_PT
dc.identifier.doi10.1016/j.jprot.2021.104274pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/21845
dc.language.isoengpt_PT
dc.subjectSpirulinapt_PT
dc.subjectcarbohydrate-active enzymes (CAZyme)pt_PT
dc.subjectweaned pigletspt_PT
dc.subjectmuscle proteomept_PT
dc.titleInfluence of dietary Spirulina inclusion and lysozyme supplementation on the longissimus lumborum muscle proteome of newly weaned pigletspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/144571/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157519/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/74495/PT
oaire.citation.titleJ Proteomicspt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameBengala Freire
person.givenNameJoão Pedro
person.identifier.ciencia-id871A-F6C8-60FE
person.identifier.orcid0000-0002-1811-4378
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication99660917-13cd-47e0-bf7d-667497ebcfbe
relation.isAuthorOfPublication.latestForDiscovery99660917-13cd-47e0-bf7d-667497ebcfbe
relation.isProjectOfPublication4c353cfa-8250-4bcf-a9d1-80b80fe7f7a4
relation.isProjectOfPublication8321c426-46ca-459c-b8bc-b6030f31a94c
relation.isProjectOfPublication2343b198-18c9-4a7d-89ee-0af634c622d2
relation.isProjectOfPublication.latestForDiscovery8321c426-46ca-459c-b8bc-b6030f31a94c

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