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Advisor(s)
Abstract(s)
Arthrospira platensis (Spirulina) is a microalga with a high content of crude protein. Its
recalcitrant cell wall limits the accessibility of the animal endogenous enzymes to its intracellular
nutrients. Enzymatic supplementation aiming to degrade cell walls could benefit microalgae digestibility.
The objective of this study was to evaluate the impact of dietary Spirulina and lysozyme supplementation
over the muscle proteome of piglets during the post-weaning stage. Thirty piglets were randomly
distributed among three diets: control (no microalga), SP (10% Spirulina) and SP+L (10% Spirulina +
0.01% lysozyme). After 4 weeks, they were sacrificed and samples of the longissimus lumborum muscle
were taken. The muscle proteome was analysed using a Tandem Mass Tag (TMT)-based quantitative
approach. A total of 832 proteins were identified. Three comparisons were computed: SP vs Ctrl, SP+L
vs Ctrl and SP+L vs SP. They had ten, four and twelve differentially abundant proteins. Glycogen
metabolism and nutrient reserves utilization are seemingly increased in the SP piglets. Structural muscle
protein synthesis increased, causing higher energy requirements in SP+L piglets. Our results demonstrate
the usefulness of proteomics to disclose the effect of dietary microalgae, whilst unveiling putative
mechanisms derived from lysozyme supplementation. Data are available via ProteomeXchange with
identifier PXD024083
Description
Keywords
Spirulina carbohydrate-active enzymes (CAZyme) weaned piglets muscle proteome
Pedagogical Context
Citation
Ribeiro DM, Martins CF, Kuleš J, Horvatić A, Guillemin N, Freire JPB, Eckersall PD, Almeida AM, Prates JAM (2021). Influence of dietary Spirulina inclusion and lysozyme supplementation on the longissimus lumborum muscle proteome of newly weaned piglets. J Proteomics. 2021 Jul 30;244:104274