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Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections

2022-09Journal article Open access
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dc.contributor.authorDardouri, Maïssa
dc.contributor.authorAljnadi, Israa M.
dc.contributor.authorDeuermeier, Jonas
dc.contributor.authorSantos, Catarina
dc.contributor.authorCosta, Fabiola
dc.contributor.authorMartin, Victor
dc.contributor.authorFernandes, Maria H.
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorBettencourt, Ana
dc.contributor.authorGomes, Pedro Sousa
dc.contributor.authorRibeiro, Isabel A. C.
dc.date.accessioned2023-09-11T17:20:22Z
dc.date.available2023-09-11T17:20:22Z
dc.date.issued2022-09
dc.date.updated2023-02-27T14:54:38Z
dc.description.abstractIn clinic there is a demand to solve the drawback of medical devices multispecies related infections. Consequently, different biomaterial surfaces, such as vascular catheters, urgently need improvement regarding their antifouling/antimicrobial properties. In this work, we covalently functionalized medical grade polydimethylsiloxane (PDMS) with antimicrobial rhamnolipids to investigate the biomaterial surface activity towards mono and dual species biofilms. Preparation of surfaces with “piranha” oxidation, followed by APTES bonding and carbodiimide reaction with rhamnolipids effectively bonded these compounds to PDMS surface as confirmed by FTIR-ATR and XPS analysis. Generated surfaces were active towards S. aureus biofilm formation showing a 4.2 log reduction while with S. epidermidis and C. albicans biofilms a reduction of 1.2 and 1.0 log reduction, respectively, was observed. Regarding dual-species testing the higher biofilm log reduction observed was 1.9. Additionally, biocompatibility was assessed by cytocompatibility towards human fibroblastic cells, low platelet activation and absence of vascular irritation. Our work not only sheds light on using covalently bonded rhamnolipids towards dual species biofilms but also highlights the biocompatibility of the obtained PDMS surfaces.pt_PT
dc.description.sponsorshipThe authors thank Fundação para a Ciência e Tecnologia (FCT) Portugal, for the financial support under the Project PTDC/BTM-SAL/29335/2017. Portugal 2020 for the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa RNEM) is also acknowledged. The authors also acknowledge the Designer Nuno Monge for the artwork, Isabel Dias Nogueira (Microlab, Instituto Superior Técnico, Lisbon University) for the technical assistance with the SEM analysis, Andreia Bento for the assistance with the UHPLC-MS equipment (iMed.Ulisboa) and Professor Maria José Ferreira (FFULisboa) for allowing the use of the automatic flash chromatography equipment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDardouri M, Aljnadi IM, Deuermeier J, Santos C, Costa F, Martin V, et al. Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections. Colloids and Surfaces B: Biointerfaces [Internet]. 1 de setembro de 2022;217:112679. Disponível em: https://www.sciencedirect.com/science/article/pii/S0927776522003629pt_PT
dc.identifier.doi10.1016/j.colsurfb.2022.112679pt_PT
dc.identifier.issn0927-7765
dc.identifier.slugcv-prod-3147589
dc.identifier.urihttp://hdl.handle.net/10451/59212
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAntiBiofilm features of Glycolipid-functionalized Silicone Catheters towards Healthcare-associated infections prevention
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0927776522003629pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAntimicrobialspt_PT
dc.subjectMedical devicespt_PT
dc.subjectPDMSpt_PT
dc.subjectAntibiofilmpt_PT
dc.subjectRhamnolipidspt_PT
dc.subjectBiosurfactantspt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectCandida albicanspt_PT
dc.subjectStaphylococcus epidermidispt_PT
dc.titleBonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infectionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAntiBiofilm features of Glycolipid-functionalized Silicone Catheters towards Healthcare-associated infections prevention
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-SAL%2F29335%2F2017/PT
oaire.citation.startPage112679pt_PT
oaire.citation.titleColloids and Surfaces B: Biointerfacespt_PT
oaire.citation.volume217pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameDiogo Gonçalves
person.familyNameCampos Simão Bettencourt
person.familyNameRibeiro
person.givenNameLídia Maria
person.givenNameAna Francisca
person.givenNameIsabel
person.identifierM-4219-2013
person.identifier163977
person.identifier.ciencia-id7211-22BA-86AD
person.identifier.ciencia-id7C1F-F932-33DC
person.identifier.ciencia-idE31A-26B8-4AC6
person.identifier.orcid0000-0002-6799-2740
person.identifier.orcid0000-0002-8498-5892
person.identifier.orcid0000-0002-7110-6393
person.identifier.ridA-6098-2014
person.identifier.scopus-author-id6603687672
person.identifier.scopus-author-id22836446000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid7211-22BA-86AD | Lídia Maria Diogo Gonçalves
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3265148d-dc2d-4601-a832-2e72f7be5662
relation.isAuthorOfPublication9068061a-d0cf-433b-a6ca-598031d0645a
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