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Advisor(s)
Abstract(s)
A fucose-containing exopolysaccharide (EPS) was produced by the bacterium Enterobacter A47 using
glycerol byproduct from the biodiesel industry. The analysis of kinetic data suggested a partially growth
associated EPS synthesis model. Although the EPS was composed of fucose, galactose and glucose at all cultivation
stages, their relative proportion has varied considerably during the run. At the beginning (24 h),
glucose was the main component (82.4 wt.%), being fucose and galactose minor components (5.0 wt.% and
10.9 wt.%, respectively), while at the end (96 h) it was composed of 26.0 wt.% fucose, 28.9 wt.% galactose
and 43.7 wt.% glucose. The acyl groups content and composition have also changed, reaching their maximum
content (19.2 wt.%) at the end of the run. Moreover, the molecular weight has increased linearly
during the run (from 8
×
105 to 5
×
106). The changes observed in EPS composition and molecular weight
have also had an impact upon the polymer’s intrinsic viscosity, as shown by its linear increase from 3.95
to 10.72 dL g−1. The results suggest that the culture might have synthesized at least two distinct EPS, with
different sugar composition and average molecular weight, which predominated at different cultivation
stages
Description
Available at ScienceDirect
Keywords
Enterobacter glycerol byproduct exopolysaccharide fucose
Pedagogical Context
Citation
"Journal of Biotechnology". ISSN 0168-1656. 156 (2011) 261-267