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Strontium hydroxyapatite and thymol nanoparticles doped in zein films as antimicrobial coatings for medical devices
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Este projeto baseia-se no desenvolvimento de revestimentos antimicrobianos para dispositivos médicos utilizando hidroxiapatite de estrôncio (SrHA) e nanopartículas de timol introduzidas em filmes de zeína. As infeções pós-operatórias, uma preocupação significativa e comum que pode levar a complicações graves de saúde, conduzem ao
objetivo principal deste estudo - a criação de uma barreira protetora que previna eficazmente tal resultado. O estudo inicia-se com a síntese de nanopartículas de timol utilizando um sistema microfluídico, escolhido pela sua precisão e eficiência na produção de sistemas de transporte com tamanho e propriedades controladas. O diâmetro hidrodinâmico e o potencial zeta das nanopartículas são medidos com precisão para garantir que cumprem os requisitos ideais de tamanho e estabilidade para uma ação antimicrobiana eficaz. A eficiência do encapsulamento é um foco principal, pois determina a forma como os agentes antimicrobianos estão integrados nas nanopartículas, um fator crucial que indica se o timol tem uma libertação sustentada no seu meio envolvente. As soluções de SrCl2, CaCl2 e Na2HPO4 são combinadas sequencialmente para obter sais de SrHA, que são integrados nos filmes, juntamente com nanopartículas de timol. Com todos os agentes incorporados, as propriedades mecânicas dos filmes são rigorosamente testadas para garantir que possuem a resistência e flexibilidade necessárias para uma futura aplicação prática em dispositivos médicos. Adicionalmente, o comportamento de degradação dos filmes é analisado para
confirmar a sua estabilidade e longevidade em condições fisiológicas. Os resultados indicam que a compatibilidade com o ambiente osteoarticular é melhorada com a incorporação de SrHA nos filmes de zeína, fortalecendo simultaneamente as suas propriedades antimicrobianas. Além disso, a biocompatibilidade dos revestimentos é testada meticulosamente para garantir que não danificam células humanas, um aspeto crítico para a aplicação segura dos revestimentos na prática clínica. Os revestimentos antimicrobianos mostram ser significativamente promissores na redução das taxas de infeção, contribuindo assim para dispositivos médicos mais seguros e eficazes, abrindo um caminho para o desenvolvimento e oferta de soluções viáveis para o desafio persistente das infeções pós-operatórias.
This project is based on the development of antimicrobial coatings for medical devices using strontium hydroxyapatite (SrHA) and thymol nanoparticles doped in zein films. Post-operative infections, a significant and common concern that can lead to severe health complications, leads to the prime objective of this study - the creation of a protective barrier that effectively prevents such outcome. The study begins with the synthesis of thymol nanoparticles using a microfluidic system, chosen for its precision and efficiency in producing delivery systems with controlled size and properties. The nanoparticles’ hydrodynamic diameter and zeta potential are precisely measured to ensure they meet the optimal size and stability requirements for effective antimicrobial action. Encapsulation efficiency is a key focus, as it determines how well antimicrobial agents are integrated within the nanoparticles, a crucial factor that indicates whether thymol has a sustained release in its surroundings. SrCl2, CaCl2 and Na2HPO4 solutions are combined sequentially to obtain SrHA salts, which are integrated in the films, alongside thymol nanoparticles. With all the agents incorporated, the mechanical properties of the films are rigorously tested to ensure they possess the necessary strength and flexibility for a future practical application on medical devices. Additionally, the degradation behaviour of the films is analysed to confirm their stability and longevity under physiological conditions. The results indicate that the compatibility with the osteo-articular environment is enhanced with the incorporation of SrHA in zein films, strengthening simultaneously their antimicrobial properties. Furthermore, the biocompatibility of the coatings is tested meticulously to ensure they do not injure human cells, a critical aspect for the safe application of the coatings in clinical practice. The antimicrobial coatings show a significant promise for reducing infection rates, thereby contributing to safer and more effective medical devices, paving the way for the development and offering of viable solutions to the persistent challenge of post-operative infections.
This project is based on the development of antimicrobial coatings for medical devices using strontium hydroxyapatite (SrHA) and thymol nanoparticles doped in zein films. Post-operative infections, a significant and common concern that can lead to severe health complications, leads to the prime objective of this study - the creation of a protective barrier that effectively prevents such outcome. The study begins with the synthesis of thymol nanoparticles using a microfluidic system, chosen for its precision and efficiency in producing delivery systems with controlled size and properties. The nanoparticles’ hydrodynamic diameter and zeta potential are precisely measured to ensure they meet the optimal size and stability requirements for effective antimicrobial action. Encapsulation efficiency is a key focus, as it determines how well antimicrobial agents are integrated within the nanoparticles, a crucial factor that indicates whether thymol has a sustained release in its surroundings. SrCl2, CaCl2 and Na2HPO4 solutions are combined sequentially to obtain SrHA salts, which are integrated in the films, alongside thymol nanoparticles. With all the agents incorporated, the mechanical properties of the films are rigorously tested to ensure they possess the necessary strength and flexibility for a future practical application on medical devices. Additionally, the degradation behaviour of the films is analysed to confirm their stability and longevity under physiological conditions. The results indicate that the compatibility with the osteo-articular environment is enhanced with the incorporation of SrHA in zein films, strengthening simultaneously their antimicrobial properties. Furthermore, the biocompatibility of the coatings is tested meticulously to ensure they do not injure human cells, a critical aspect for the safe application of the coatings in clinical practice. The antimicrobial coatings show a significant promise for reducing infection rates, thereby contributing to safer and more effective medical devices, paving the way for the development and offering of viable solutions to the persistent challenge of post-operative infections.
Descrição
Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, 2024, Universidade de Lisboa, Faculdade de Farmácia.
Palavras-chave
Strontium hydroxyapatite Thymol Zein films Antimicrobial coatings Medical devices Mestrado Integrado - 2024