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Metabarcoding reveals southern hemisphere fungal endophytes within wood of cultivated Proteaceae in Portugal

2021Artigo científico Acesso aberto
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dc.contributor.authorMigliorini, Duccio
dc.contributor.authorMessal, Mandy
dc.contributor.authorSantini, Alberto
dc.contributor.authorRamos, Ana Paula
dc.contributor.authorTalhinhas, Pedro
dc.contributor.authorWingfield, Michael J.
dc.contributor.authorBurgess, Treena
dc.date.accessioned2021-09-27T13:44:47Z
dc.date.available2021-09-27T13:44:47Z
dc.date.issued2021
dc.description.abstractEndophytic fungal hitch-hikers have been difficult to detect in the past, and have potentially spread these latent pathogens via the global plant trade. The African genera Protea, Leucospermum and Leucadendron, commercially referred to as proteas, form the basis of a global flower production industry. The largest producers of proteas are Australia and South Africa, followed by Portugal and Spain. In the 1990s propagation material from South Africa was used to establish protea orchards in Portugal. We utilized metabarcoding to determine if this plant trade has carried host-specific fungal pathogens to a new environment. Wood samples collected from asymtomatic twigs from Portuguese farms, where propagation material had been imported from South Africa, was compared to material from South African farms that originally produced and supplied rooted and unrooted cuttings. DNA metabarcoding, using fungal-specific primers for the ITS2 gene region, produced 1237 OTUs. Focusing only on known pathogens of protea, we found that the Portuguese orchards contained fungal disease agents associated with Proteaceae or other plant families from the Southern Hemisphere. Our sampling technique could be used by agencies and applied to other plant material and pathogens to reduce the spread of pathogenspt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEur J Plant Pathol (2021) 160:173–184pt_PT
dc.identifier.doihttps://doi.org/10.1007/s10658-021-02233-8pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/22037
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectProteaceaept_PT
dc.subjectfungal endophytespt_PT
dc.subjectSouth Africapt_PT
dc.subjectPortugalpt_PT
dc.subjectwoodpt_PT
dc.subjectmetabarcodingpt_PT
dc.titleMetabarcoding reveals southern hemisphere fungal endophytes within wood of cultivated Proteaceae in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleEur J Plant Patholpt_PT
person.familyNameRamos
person.familyNameTalhinhas
person.givenNameAna Paula
person.givenNamePedro
person.identifier171875
person.identifier.ciencia-id9914-C6BD-6554
person.identifier.ciencia-id5A1E-9983-ADD5
person.identifier.orcid0000-0001-5974-7481
person.identifier.orcid0000-0001-6694-7502
person.identifier.ridJ-4784-2013
person.identifier.scopus-author-id57199666135
person.identifier.scopus-author-id6506057702
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication43f0808f-c48e-442b-b6d4-7f6822988fc0
relation.isAuthorOfPublicationeba8a4d6-04bf-4aa6-8a7d-e0bcef764ae7
relation.isAuthorOfPublication.latestForDiscoveryeba8a4d6-04bf-4aa6-8a7d-e0bcef764ae7

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