A carregar...
Publicação
Metabarcoding reveals southern hemisphere fungal endophytes within wood of cultivated Proteaceae in Portugal
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 625.57 KB | Adobe PDF |
Orientador(es)
Resumo(s)
Endophytic fungal hitch-hikers have been
difficult to detect in the past, and have potentially spread
these latent pathogens via the global plant trade. The
African genera Protea, Leucospermum and
Leucadendron, commercially referred to as proteas,
form the basis of a global flower production industry.
The largest producers of proteas are Australia and South Africa, followed by Portugal and Spain. In the 1990s
propagation material from South Africa was used to
establish protea orchards in Portugal. We utilized
metabarcoding to determine if this plant trade has carried
host-specific fungal pathogens to a new environment.
Wood samples collected from asymtomatic twigs
from Portuguese farms, where propagation material had
been imported from South Africa, was compared to
material from South African farms that originally produced
and supplied rooted and unrooted cuttings. DNA
metabarcoding, using fungal-specific primers for the
ITS2 gene region, produced 1237 OTUs. Focusing only
on known pathogens of protea, we found that the Portuguese
orchards contained fungal disease agents associated
with Proteaceae or other plant families from the
Southern Hemisphere. Our sampling technique could be
used by agencies and applied to other plant material and
pathogens to reduce the spread of pathogens
Descrição
Palavras-chave
Proteaceae fungal endophytes South Africa Portugal wood metabarcoding
Contexto Educativo
Citação
Eur J Plant Pathol (2021) 160:173–184
Editora
Springer