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Nisin influence on the antimicrobial resistance ability of canine oral enterococci

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dc.contributor.authorCunha, Eva
dc.contributor.authorJanela, Rita
dc.contributor.authorCosta, Margarida
dc.contributor.authorTavares, Luís
dc.contributor.authorVeiga, Ana Salomé
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2021-04-01T15:30:32Z
dc.date.available2021-04-01T15:30:32Z
dc.date.issued2020
dc.description© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0).pt_PT
dc.description.abstractPeriodontal disease (PD) is one of the most common diseases in dogs. Although previous studies have shown the potential of the antimicrobial peptide nisin for PD control, there is no information regarding its influence in the development of antimicrobial resistance or horizontal gene transfer (HGT). Nisin’s mutant prevention concentration (MPC) and selection window (MSW) were determined for a collection of canine oral enterococci. Isolates recovered after the determination of the MPC values were characterized for their antimicrobial profile and its nisin minimum inhibitory and bactericidal concentrations. The potential of vanA HGT between Enterococcus faecium CCGU36804 and nine clinical canine staphylococci and enterococci was evaluated. Nisin MPC values ranged from 400 to more than 600 µg/mL. In comparison with the original enterococci collection, the isolates recovered after the determination of the nisin MPC showed increased resistance towards amoxicillin/clavulanate (5%), vancomycin (5%), enrofloxacin (10%), gentamicin (10%) and imipenem (15%). The HGT of vanA gene was not observed. This work showed that nisin selective pressure may induce changes in the bacteria’s antimicrobial resistance profile but does not influence horizontal transfer of vanA gene. To our knowledge, this is the first report of nisin’s MPC and MSW determination regarding canine enterococci.pt_PT
dc.description.sponsorshipAuthors would like to acknowledge the Foundation for Science and Technology (Eva Cunha PhD fellowship SFRH/BD/131384/2017) and to CIISA–Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Project UIDB/00276/2020 (Funded by FCT), for financial support of this work.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntibiotics (Basel). 2020 Dec 10;9(12):890pt_PT
dc.identifier.doi10.3390/antibiotics9120890pt_PT
dc.identifier.eissn2079-6382
dc.identifier.urihttp://hdl.handle.net/10451/47206
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPrevention of periodontal disease in dogs: an experimental approach using an antimicrobial peptide
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relation.publisherversionhttps://www.mdpi.com/journal/antibioticspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNisinpt_PT
dc.subjectMutant prevention concentrationpt_PT
dc.subjectMutant selection windowpt_PT
dc.subjectAntimicrobial susceptibility testingpt_PT
dc.subjectHorizontal gene transferpt_PT
dc.titleNisin influence on the antimicrobial resistance ability of canine oral enterococcipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePrevention of periodontal disease in dogs: an experimental approach using an antimicrobial peptide
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F131384%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.citation.issue12pt_PT
oaire.citation.startPage890pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVeiga
person.givenNameAna Salome
person.identifier.orcid0000-0002-9892-2243
person.identifier.scopus-author-id56745037100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoverye4303354-5eaa-4d48-8198-de1adc9beb19
relation.isProjectOfPublication0290c3d4-bab7-492d-bfe9-413aca3dfeae
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