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UV-C irradiation-based inactivation of SARS-CoV-2 in contaminated porous and non-porous surfaces

2022Artigo científico Acesso restrito
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dc.contributor.authorTomás, Ana Luísa
dc.contributor.authorReichel, Anna
dc.contributor.authorSilva, Patrícia M.
dc.contributor.authorSilva, Pedro G.
dc.contributor.authorPinto, João
dc.contributor.authorCalado, Inês
dc.contributor.authorCampos, Joana
dc.contributor.authorSilva, Ilídio
dc.contributor.authorMachado, Vasco
dc.contributor.authorLaranjeira, Roberto
dc.contributor.authorAbreu, Paulo
dc.contributor.authorMendes, Paulo
dc.contributor.authorSedrine, Nabiha Ben
dc.contributor.authorSantos, Nuno C.
dc.date.accessioned2022-09-09T14:59:09Z
dc.date.available2022-09-09T14:59:09Z
dc.date.issued2022
dc.description© 2022 Elsevier B.V. All rights reserved.pt_PT
dc.description.abstractThe SARS-CoV-2 pandemic emphasized effective cleaning and disinfection of common spaces as an essential tool to mitigate viral transmission. To address this problem, decontamination technologies based on UV-C light are being used. Our aim was to generate coherent and translational datasets of effective UV-C-based SARS-CoV-2 inactivation protocols for the application on surfaces with different compositions. Virus infectivity after UV-C exposure of several porous (bed linen, various types of upholstery, synthetic leather, clothing) and non-porous (types of plastic, stainless steel, glass, ceramics, wood, vinyl) materials was assessed through plaque assay using a SARS-CoV-2 clinical isolate. Studies were conducted under controlled environmental conditions with a 254-nm UV-C lamp and irradiance values quantified using a 254 nm-calibrated sensor. From each material type (porous/non-porous), a product was selected as a reference to assess the decrease of infectious virus particles as a function of UV-C dose, before testing the remaining surfaces with selected critical doses. Our data show that UV-C irradiation is effectively inactivating SARS-CoV-2 on both material types. However, an efficient reduction in the number of infectious viral particles was achieved much faster and at lower doses on non-porous surfaces. The treatment effectiveness on porous surfaces was demonstrated to be highly variable and composition-dependent. Our findings will support the optimization of UV-C-based technologies, enabling the adoption of effective customizable protocols that will help to ensure higher antiviral efficiencies.pt_PT
dc.description.sponsorshipThis work was developed within the UVtizer project, constituted by the consortium Castros, MATGLOW, CeNTI & iMM, supported by COMPETE 2020 and Portugal 2020 through ERDF (grant number POCI-01-02B7-FEDER-062110). P.M.S. acknowledges fellowship SFRH/BD/118413/2016 from Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Photochem Photobiol B. 2022 Sep;234:112531pt_PT
dc.identifier.doi10.1016/j.jphotobiol.2022.112531pt_PT
dc.identifier.eissn1873-2682
dc.identifier.issn1011-1344
dc.identifier.urihttp://hdl.handle.net/10451/54423
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-02B7-FEDER-062110pt_PT
dc.relationLipid-tagged peptides as entry inhibitors for respiratory viruses
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/journal-of-photochemistry-and-photobiology-b-biologypt_PT
dc.subjectCOVID-19pt_PT
dc.subjectNon-porous surfacespt_PT
dc.subjectPorous surfacespt_PT
dc.subjectSARS-CoV-2pt_PT
dc.subjectUV-C irradiationpt_PT
dc.subjectViral inactivationpt_PT
dc.titleUV-C irradiation-based inactivation of SARS-CoV-2 in contaminated porous and non-porous surfacespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/118413/2016
oaire.awardTitleLipid-tagged peptides as entry inhibitors for respiratory viruses
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F118413%2F2016/PT
oaire.citation.startPage112531pt_PT
oaire.citation.titleJournal of Photochemistry and Photobiology B: Biologypt_PT
oaire.citation.volume234pt_PT
oaire.fundingStreamOE
person.familyNameTomás
person.familyNameReichel
person.familyNameM. Silva
person.familyNameSantos
person.givenNameAna Luísa
person.givenNameAnna
person.givenNamePatrícia
person.givenNameNuno
person.identifier.ciencia-id8B1A-F73C-BE28
person.identifier.ciencia-id1A18-2AAE-83A2
person.identifier.ciencia-idCD13-5E3A-A3C5
person.identifier.orcid0000-0003-2254-6154
person.identifier.orcid0000-0003-4968-1428
person.identifier.orcid0000-0001-5055-785X
person.identifier.orcid0000-0002-0580-0475
person.identifier.ridN-7248-2013
person.identifier.scopus-author-id57191879236
person.identifier.scopus-author-id55940818300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication83c5ad90-61fe-4d44-b589-5cf8585c2c91
relation.isAuthorOfPublication72b963e6-e21d-4438-814f-a232bad1d19e
relation.isAuthorOfPublication6b1dbc54-f4cf-497e-85f5-e2d225611718
relation.isAuthorOfPublication4656d912-5a2d-4c80-8921-2bcba3aa441a
relation.isAuthorOfPublication.latestForDiscovery83c5ad90-61fe-4d44-b589-5cf8585c2c91
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