Publication
Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
| dc.contributor.author | Sousa, Ana E. | |
| dc.contributor.author | Chaves, Ana F. | |
| dc.contributor.author | Doroana, Manuela | |
| dc.contributor.author | Antunes, Francisco | |
| dc.contributor.author | Victorino, Rui M. M. | |
| dc.date.accessioned | 2014-06-04T15:45:37Z | |
| dc.date.available | 2014-06-04T15:45:37Z | |
| dc.date.issued | 2000 | |
| dc.description | Copyright © 2000, Elsevier | eng |
| dc.description.abstract | Cytokine imbalances play a major role in HIV immunopathogenesis. This study analyzes simultaneously the frequency of cytokine-producing cells at the single cell level by flow cytometry and the disturbances in cytokine secretion assessed by ELISA in a cohort of asymptomatic HIV1 patients in different stages of CD4 depletion and during antiretroviral therapy (HAART). Early in the disease, there is an increased frequency of IFN-γ lymphocytes and bulk IFN-γ+ production, in parallel with a reduced proportion of IL4+ cells and IL4 secreted. The two IL4 measurements are significantly correlated. No such correlation was found for IFN-γ, which is consistent with a large variation in the amount of IFN-γ released per individual cell. Moreover, HAART was associated with a reduction to normal levels in the bulk IFN-γ secretion concomitant with a persistency of the overexpanded IFN-γ+ cell subset in the peripheral blood. This study emphasizes the importance of using a conjoint approach to assess the cytokine network in trials of antiretroviral and/or immune-based therapies to avoid missing significant effects which are possibly relevant in the clinical setting. | eng |
| dc.description.sponsorship | This work was supported by grants from “Ministério da Ciência e Tecnologia–Praxis XXI” and from “Comissão Nacional de Luta Contra a SIDA–Ministério da Saúde” to RMMV. AES received a scholarship from Praxis and AFC received a scholarship from CNLCS. | eng |
| dc.identifier.citation | Clinical Immunology, Vol. 97, No. 2, November, pp. 162–170, 2000 | por |
| dc.identifier.issn | 1521-6616 | |
| dc.identifier.uri | http://dx.doi.org/10.1006/clim.2000.4930 | |
| dc.identifier.uri | http://hdl.handle.net/10451/11129 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | eng |
| dc.subject | HIV | eng |
| dc.subject | Cytokines | eng |
| dc.subject | Antiretroviral therapy | eng |
| dc.subject | Immunological reconstitution | eng |
| dc.subject | Intracellular cytokine detection | eng |
| dc.title | Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 170 | por |
| oaire.citation.startPage | 162 | por |
| oaire.citation.title | Clinical Immunology | por |
| rcaap.rights | closedAccess | por |
| rcaap.type | article | por |