A carregar...
Publicação
Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 93.96 KB | Adobe PDF |
Orientador(es)
Resumo(s)
Cytokine imbalances play a major role in HIV immunopathogenesis.
This study analyzes simultaneously the frequency of cytokine-producing cells at the single cell level by flow cytometry and the disturbances in cytokine secretion assessed by ELISA in a cohort of asymptomatic HIV1 patients in different stages of CD4 depletion and during antiretroviral therapy (HAART).
Early in the disease, there is an increased frequency of IFN-γ lymphocytes and bulk IFN-γ+ production, in
parallel with a reduced proportion of IL4+ cells and IL4 secreted. The two IL4 measurements are significantly
correlated. No such correlation was found for IFN-γ, which is consistent with a large variation in the amount of IFN-γ released per individual cell. Moreover, HAART was associated with a reduction to normal levels in the bulk IFN-γ secretion concomitant with a persistency of the overexpanded IFN-γ+ cell subset in the peripheral blood. This study emphasizes the importance of using a conjoint approach to assess the cytokine network in trials of antiretroviral and/or immune-based therapies to avoid missing significant effects which are possibly relevant in the clinical setting.
Descrição
Copyright © 2000, Elsevier
Palavras-chave
HIV Cytokines Antiretroviral therapy Immunological reconstitution Intracellular cytokine detection
Contexto Educativo
Citação
Clinical Immunology, Vol. 97, No. 2, November, pp. 162–170, 2000
Editora
Elsevier