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Platinum nanoparticle-based microreactors protect against the behavioral and neurobiological consequences of chronic stress exposure

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dc.contributor.authorHirata, Rafael Y. S.
dc.contributor.authorOliveira, Roberto N.
dc.contributor.authorSilva, Mariana S. C. F.
dc.contributor.authorArmada-Moreira, Adam
dc.contributor.authorVaz, Sandra H.
dc.contributor.authorRibeiro, Filipa
dc.contributor.authorSebastião, Ana M
dc.contributor.authorLemes, Jéssica A.
dc.contributor.authorde Andrade, José S.
dc.contributor.authorRosário, Bárbara A.
dc.contributor.authorCéspedes, Isabel C.
dc.contributor.authorViana, Milena B.
dc.date.accessioned2022-12-06T14:41:36Z
dc.date.available2022-12-06T14:41:36Z
dc.date.issued2022
dc.description© 2022 Elsevier Inc. All rights reservedpt_PT
dc.description.abstractExcitotoxicity is described as the exacerbated activation of glutamate AMPA and NMDA receptors that leads to neuronal damage, and ultimately to cell death. Astrocytes are responsible for the clearance of 80-90% of synaptically released glutamate, preventing excitotoxicity. Chronic stress renders neurons vulnerable to excitotoxicity and has been associated to neuropsychiatric disorders, i.e., anxiety. Microreactors containing platinum nanoparticles (Pt-NP) and glutamate dehydrogenase have shown in vitro activity against excitotoxicity. The purpose of the present study was to investigate the in vivo effects of these microreactors on the behavioral and neurobiological effects of chronic stress exposure. Rats were either unstressed or exposed for 2 weeks to an unpredictable chronic mild stress paradigm (UCMS), administered intra-ventral hippocampus with the microreactors (with or without the blockage of astrocyte functioning), and seven days later tested in the elevated T-maze (ETM; Experiment 1). The ETM allows the measurement of two defensive responses, avoidance and escape, in terms of psychopathology respectively related to generalized anxiety and panic disorder. Locomotor activity in an open field was also measured. Since previous evidence shows that stress inhibits adult neurogenesis, we evaluated the effects of the different treatments on the number of cells expressing the marker of migrating neuroblasts doublecortin (DCX) in the dorsal and ventral hippocampus (Experiment 2). Results showed that UCMS induces anxiogenic effects, increases locomotion, and decreases the number of DCX cells in the dorsal and ventral hippocampus, effects that were counteracted by microreactor administration. This is the first study to demonstrate the in vivo efficacy of Pt-NP against the behavioral and neurobiological effects of chronic stress exposure.pt_PT
dc.description.sponsorshipThis study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, number 2019/26777-8), Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES PRINT 803269P), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação para a Ciência e para a Tecnologia (FCT) (PTDC/BTM-SAL/32147/2017), and 2020-WIDESPREAD-05-2017-Twinning (EpiEpinet) under grant agreement No. 952455.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBrain Res Bull. 2022 Nov;190:1-11pt_PT
dc.identifier.doi10.1016/j.brainresbull.2022.09.004pt_PT
dc.identifier.eissn1873-2747
dc.identifier.issn0361-9230
dc.identifier.urihttp://hdl.handle.net/10451/55357
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAstrosomes: Artificial Astrocytes as a Novel Approach to Regulate Neuronal Communication
dc.relationEpileptogenesis and Epilepsy Network: from genes, synapses and circuits to pave the way for novel drugs and strategies
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/brain-research-bulletinpt_PT
dc.subjectBehaviorpt_PT
dc.subjectChronic stresspt_PT
dc.subjectDCXpt_PT
dc.subjectExcitotoxicitypt_PT
dc.subjectNeurogenesispt_PT
dc.subjectPlatinum nanoparticle-based microreactorspt_PT
dc.titlePlatinum nanoparticle-based microreactors protect against the behavioral and neurobiological consequences of chronic stress exposurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAstrosomes: Artificial Astrocytes as a Novel Approach to Regulate Neuronal Communication
oaire.awardTitleEpileptogenesis and Epilepsy Network: from genes, synapses and circuits to pave the way for novel drugs and strategies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-SAL%2F32147%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/952455/EU
oaire.citation.endPage11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleBrain Research Bulletinpt_PT
oaire.citation.volume190pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamH2020
person.familyNameHenriques Vaz
person.familyNameRibeiro
person.familyNameSebastião
person.givenNameSandra Cristina
person.givenNameFilipa
person.givenNameAna M
person.identifier.ciencia-id0E1C-952D-61BE
person.identifier.ciencia-id6916-485D-36C3
person.identifier.ciencia-idF112-55E8-E37E
person.identifier.orcid0000-0003-4258-9397
person.identifier.orcid0000-0002-4006-7328
person.identifier.orcid0000-0001-9030-6115
person.identifier.ridS-1079-2018
person.identifier.scopus-author-id7004409879
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb282e0fa-69eb-4079-8752-b7dd914f7745
relation.isAuthorOfPublication45abc672-83ac-42b0-97d5-06f5633530f8
relation.isAuthorOfPublication304abd7f-071b-4447-a8a3-4aa5f0547141
relation.isAuthorOfPublication.latestForDiscoveryb282e0fa-69eb-4079-8752-b7dd914f7745
relation.isProjectOfPublication1945faf0-0fa5-48d5-90b4-00ae1f0fef66
relation.isProjectOfPublication71376ce5-3732-4d41-b053-3dad707d6191
relation.isProjectOfPublication.latestForDiscovery1945faf0-0fa5-48d5-90b4-00ae1f0fef66

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