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Efeito da contaminação salivar na adesão à dentina com adesivos universais
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Objetivo: Avaliar a influência da contaminação salivar após polimerização de um adesivo universal e comparar a eficácia de dois diferentes métodos de descontaminação de saliva e reaplicação do adesivo, na resistência adesiva sob forças de corte à dentina, após 24 horas e 6 meses. Materiais e métodos: 50 molares íntegros foram seccionados, de forma a obter duas fatias de dentina por dente. Estas foram distribuídas aleatoriamente em 5 grupos (SC – sem contaminação, A – descontaminação com água, A+Ad – descontaminação com água seguida de reaplicação do adesivo, E – descontaminação com etanol, E+Ad – descontaminação com etanol seguida de reaplicação do adesivo), de onde foram retirados espécimes para o ensaio de resistência adesiva das 24 horas e dos 6 meses. Após aplicação do sistema adesivo (Scotchbond UniversalTM) e respetivo protocolo experimental, foi testada a resistência adesiva dos espécimes até à fratura. Seguidamente, o tipo de falha de união foi observado. Foi também realizado o estudo ultramorfológico da interface adesiva através de MEV. Os resultados obtidos foram sujeitos a testes não-paramétricos de acordo com o método Kruskal-Wallis, seguidos de testes de Mann-Whitney, de forma a comparar as diferenças entre grupos, com um nível de significância de 5%. Resultados: Os grupos sem contaminação, quer às 24 horas, quer aos 6 meses, apresentaram valores superiores de resistência adesiva (p<0,001), independentemente do método de descontaminação subsequente. A avaliação dos grupos experimentais com contaminação apresentou diferenças estatisticamente significativas (p<0,001), após 6 meses de envelhecimento, relativamente à reaplicação do adesivo, tendo este grupo apresentado valores mais elevados de adesão. O tipo de falha de união mais observada foi a falha adesiva. Conclusão: A contaminação salivar após a polimerização do adesivo universal mostrou-se prejudicial nos valores de resistência adesiva. A reaplicação do adesivo após descontaminação, apesar de não recuperar as forças de adesão, apresentou melhores resultados após 6 meses de envelhecimento.
Purpose: The aim of this study was to determine the influence of salivary contamination after curing of a universal adhesive and compare the effectiveness of two different decontamination procedures and reapplication of adhesive, on dentin bond strength, after 24 hours and 6 months. Methods: 50 sound molars were sectioned, in order to obtain two slices of dentin in each tooth. The specimens were randomly distributed into five groups (SC – no contamination; A – decontamination with water; A+Ad – decontamination with water and reapplication of adhesive; E – decontamination with ethanol; E+Ad – decontamination with ethanol and reapplication of adhesive). In which group were removed specimens for 24 hours and 6 months strength test. After application of the adhesive system (Scotchbond UniversalTM) and respective experimental protocol, the shear bond strength was tested until fracture. The type of bond failure was observed. It was also carried out the ultra-morphological study of adhesive interface through SEM. The data were statistically analysed using non-parametric Kruskal-Wallis and Mann-Whitney tests to compare differences between groups. Statistical testing was performed at a 5% level of significance. Results: Groups without contamination, either at 24 hours or at 6 months, presented higher SBS values, regardless of the subsequent decontamination method (p<0,001). The evaluation of experimental groups with contamination showed statistically differences in groups with reapplication of adhesive, after 6 months of aging (p<0,001). The most observed type of failure was adhesive. Conclusion: When saliva contamination occurs after curing of universal adhesive, it seems that significantly decreases the bond strength of the adhesive to dentin. The reapplication of adhesive after decontamination do not recovered the adhesion forces, but presented better results after 6 months of aging.
Purpose: The aim of this study was to determine the influence of salivary contamination after curing of a universal adhesive and compare the effectiveness of two different decontamination procedures and reapplication of adhesive, on dentin bond strength, after 24 hours and 6 months. Methods: 50 sound molars were sectioned, in order to obtain two slices of dentin in each tooth. The specimens were randomly distributed into five groups (SC – no contamination; A – decontamination with water; A+Ad – decontamination with water and reapplication of adhesive; E – decontamination with ethanol; E+Ad – decontamination with ethanol and reapplication of adhesive). In which group were removed specimens for 24 hours and 6 months strength test. After application of the adhesive system (Scotchbond UniversalTM) and respective experimental protocol, the shear bond strength was tested until fracture. The type of bond failure was observed. It was also carried out the ultra-morphological study of adhesive interface through SEM. The data were statistically analysed using non-parametric Kruskal-Wallis and Mann-Whitney tests to compare differences between groups. Statistical testing was performed at a 5% level of significance. Results: Groups without contamination, either at 24 hours or at 6 months, presented higher SBS values, regardless of the subsequent decontamination method (p<0,001). The evaluation of experimental groups with contamination showed statistically differences in groups with reapplication of adhesive, after 6 months of aging (p<0,001). The most observed type of failure was adhesive. Conclusion: When saliva contamination occurs after curing of universal adhesive, it seems that significantly decreases the bond strength of the adhesive to dentin. The reapplication of adhesive after decontamination do not recovered the adhesion forces, but presented better results after 6 months of aging.
Descrição
Tese de mestrado, Medicina Dentária, Universidade de Lisboa, Faculdade de Medicina Dentária, 2018
Palavras-chave
Saúde oral Adesão da dentina Saliva Teses de mestrado - 2018