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Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo

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dc.contributor.authorMoreira, Carolina G. A.
dc.contributor.authorJacinto, Antonio
dc.contributor.authorPrag, Soren
dc.date.accessioned2021-10-18T11:06:25Z
dc.date.available2021-10-18T11:06:25Z
dc.date.issued2013
dc.description© 2013. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.pt_PT
dc.description.abstractCell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of three-dimensional cell culture and in vivo systems has shown some differences with what was previously believed to be well-established cell migration mechanisms, suggesting that two-dimensional cell motility would be a poor predictor of in vivo behaviour. Drosophila is a widely recognized model organism to study developmental and homeostatic processes and has been widely used to investigate cell migration. Here, we focus on the migration of small groups of pupal hemocytes that accumulate during larval stages in dorsal patches. We show that integrins, and other known nascent adhesion-related proteins such as Rhea and Fermitin 1, are crucial for this process and that their depletion does not affect polarization in response to environmental cues. We also present evidence for the importance of adhesion maturation-related proteins in hemocyte migration, namely Zyxin. Zyxin depletion in hemocytes leads to a significant increase of cell speed without affecting their response to a chemotactic cue. This is the first report of a systematic analysis using Drosophila melanogaster hemocytes to study adhesion-related proteins and their function in cell migration in vivo. Our data point to mechanisms of cell migration similar to those described in three-dimensional in vitro systems and other in vivo model organisms.pt_PT
dc.description.sponsorshipThe work was supported by a fellowship (SFRH/BD/62345/2009) to C.G.A.M. and a grant (PTDC/SAU-OBD/101259/2008) from Fundação para a Ciência e Tecnologia, and a European Research Council Starting Grant (2007-StG-208631).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiol Open. 2013 Jun 6;2(8):795-801pt_PT
dc.identifier.doi10.1242/bio.20134564pt_PT
dc.identifier.eissn2046-6390
dc.identifier.urihttp://hdl.handle.net/10451/49920
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherThe Company of Biologistspt_PT
dc.relation2007-StG-208631pt_PT
dc.relationTHE FUNCTION OF INTEGRINS IN DROSOPHILA HAEMOCYTE MIGRATION
dc.relationFunction of Integrins in Drosophila Haemocyte Migration
dc.relation.publisherversionhttps://journals.biologists.com/biopt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDrosophilapt_PT
dc.subjectHemocytept_PT
dc.subjectIntegrinpt_PT
dc.subjectMigrationpt_PT
dc.titleDrosophila integrin adhesion complexes are essential for hemocyte migration in vivopt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/62345/2009
oaire.awardNumberPTDC/SAU-OBD/101259/2008
oaire.awardTitleTHE FUNCTION OF INTEGRINS IN DROSOPHILA HAEMOCYTE MIGRATION
oaire.awardTitleFunction of Integrins in Drosophila Haemocyte Migration
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F62345%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F101259%2F2008/PT
oaire.citation.endPage801pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage795pt_PT
oaire.citation.titleBiology Openpt_PT
oaire.citation.volume2pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione528be3d-0bb5-4ddd-bd80-5b5f207c5790
relation.isProjectOfPublication79cfd6d4-6674-48f4-a996-4c97756e1f30
relation.isProjectOfPublication.latestForDiscovery79cfd6d4-6674-48f4-a996-4c97756e1f30

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