Publication
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model
| dc.contributor.author | Neves Coelho, Sara | |
| dc.contributor.author | Eleutério, Rute P. | |
| dc.contributor.author | Enguita, Francisco J. | |
| dc.contributor.author | Neves, Vera | |
| dc.contributor.author | Castanho, Miguel A. R. B. | |
| dc.date.accessioned | 2018-04-02T14:47:34Z | |
| dc.date.available | 2018-04-02T14:47:34Z | |
| dc.date.issued | 2017 | |
| dc.description | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | pt_PT |
| dc.description.abstract | The capacity to transport therapeutic molecules across the blood–brain barrier (BBB) represents a breakthrough in the development of tools for the treatment of many central nervous system (CNS)-associated diseases. The BBB, while being protective against infectious agents, hinders the brain uptake of many drugs. Hence, finding safe shuttles able to overcome the BBB is of utmost importance. Herein, we identify a new BBB-translocating peptide with unique properties. For years it was thought that cationic sequences were mandatory for a cell-penetrating peptide (CPP) to achieve cellular internalization. Despite being anionic at physiological pH, PepNeg (sequence (SGTQEEY) is an efficient BBB translocator that is able to carry a large cargo (27 kDa), while maintaining BBB integrity. In addition, PepNeg is able to use two distinct methods of translocation, energy-dependent and -independent, suggesting that direct penetration might occur when low concentrations of peptide are presented to cells. The discovery of this new anionic trans-BBB peptide allows the development of new delivery systems to the CNS and contributes to the need to rethink the role of electrostatic attraction in BBB-translocation | pt_PT |
| dc.description.sponsorship | The authors thank the Portuguese Funding Agency, Fundação para a Ciência e a Tecnologia, FCT IP, for financial support (grants SFRH/BPD/94466/2013; PTDC/BBBNAN/1578/2014) and Marie Sklodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE), call 20-MSCA-RISE-2014 (grant agreement H20 644167—INPACT). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Molecules 2017, 22, 1753 | pt_PT |
| dc.identifier.doi | doi:10.3390/molecules22101753 | pt_PT |
| dc.identifier.issn | 1420-3049 | |
| dc.identifier.uri | http://hdl.handle.net/10451/32538 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | PTDC/BBBNAN/1578/2014 | pt_PT |
| dc.relation | Peptides for blood-brain barrier transmigration and drug delivery - novel therapies for the central nervous system CNS | |
| dc.relation.publisherversion | http://www.mdpi.com/journal/molecules | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Cell-penetrating peptide | pt_PT |
| dc.subject | Blood–brain barrier | pt_PT |
| dc.subject | Anionic peptide | pt_PT |
| dc.subject | Drug-delivery systems | pt_PT |
| dc.title | A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Peptides for blood-brain barrier transmigration and drug delivery - novel therapies for the central nervous system CNS | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F94466%2F2013/PT | |
| oaire.citation.issue | 10 | pt_PT |
| oaire.citation.title | Molecules | pt_PT |
| oaire.citation.volume | 22 | pt_PT |
| person.familyName | Neves Coelho | |
| person.givenName | Sara | |
| person.identifier.orcid | 0000-0003-2411-833X | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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