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NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning

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dc.contributor.authorRathore, Om Singh
dc.contributor.authorSilva, Rui D
dc.contributor.authorAscensão-Ferreira, Mariana
dc.contributor.authorMatos, Ricardo
dc.contributor.authorCarvalho, Célia
dc.contributor.authorMarques, Bruno
dc.contributor.authorTiago, Margarida N.
dc.contributor.authorPrudêncio, Pedro
dc.contributor.authorAndrade, Raquel P.
dc.contributor.authorRoignant, Jean-Yves
dc.contributor.authorBarbosa-Morais, Nuno
dc.contributor.authorMartinho, Rui Goncalo
dc.date.accessioned2021-06-04T14:56:36Z
dc.date.available2021-06-04T14:56:36Z
dc.date.issued2020
dc.description© 2020 Rathore et al. This article is distributed exclusively by the RNASociety for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.pt_PT
dc.description.abstractThe NineTeen Complex (NTC), also known as pre-mRNA-processing factor 19 (Prp19) complex, regulates distinct spliceosome conformational changes necessary for splicing. During Drosophila midblastula transition, splicing is particularly sensitive to mutations in NTC-subunit Fandango, which suggests differential requirements of NTC during development. We show that NTC-subunit Salsa, the Drosophila ortholog of human RNA helicase Aquarius, is rate-limiting for splicing of a subset of small first introns during oogenesis, including the first intron of gurken Germline depletion of Salsa and splice site mutations within gurken first intron impair both adult female fertility and oocyte dorsal-ventral patterning, due to an abnormal expression of Gurken. Supporting causality, the fertility and dorsal-ventral patterning defects observed after Salsa depletion could be suppressed by the expression of a gurken construct without its first intron. Altogether, our results suggest that one of the key rate-limiting functions of Salsa during oogenesis is to ensure the correct expression and efficient splicing of the first intron of gurken mRNA. Retention of gurken first intron compromises the function of this gene most likely because it undermines the correct structure and function of the transcript 5'UTR.pt_PT
dc.description.sponsorshipThe Microscopy Unit was partially supported by Portuguese national funding (FCT: PPBI-POCI-01-0145-FEDER-022122). This work was developed with the support of the research infrastructure Congento (project LISBOA-01-0145-FEDER-022170). R.G.M. is supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia (FCT grant refs. PTDC/BEX-BID/0395/2014, PTDC/BIA-BID/28441/2017, and UID/BIM/04773/2013 CBMR 1334). Nuno L. Barbosa-Morais is supported by European Molecular Biology Organization (EMBO) Installation Grant (3057), Investigador FCT Starting Grant (IF/00595/2014), UID/BIM/50005/2019, the project funded by Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado and iMM Lisboa start-up funds. O.S.R. and B.M. were supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia, respectively, PD/BD/52428/2013 and PD/BD/128342/2017, within the scope of the ProRegeM PhD program (ref. PD/00117/2012, CRM:0027030). O.S.R. was also supported by a Federation of European Biochemical societies (FEBS) short-term fellowship. M.A.-F. is supported by Fundação para a Ciência e Tecnologia (FCT) PhD fellowship (PD/BD/128283/2017) and Fundação AstraZeneca. R.D.S. is supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia, ref. DL 57/2016/CP1361/CT0019.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRNA. 2020 Dec;26(12):1935-1956pt_PT
dc.identifier.doi10.1261/rna.077446.120pt_PT
dc.identifier.eissn1469-9001
dc.identifier.issn1355-8382
dc.identifier.urihttp://hdl.handle.net/10451/48334
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCold Spring Harbor Laboratory Presspt_PT
dc.relationPPBI-POCI-01-0145-FEDER-022122pt_PT
dc.relationLISBOA-01-0145-FEDER-022170pt_PT
dc.relationIF/00595/2014pt_PT
dc.relationInstituto de Medicina Molecular
dc.relationREGULATION OF PRE-MRNA SPLICING DURING DROSOPHILA DEVELOPMENT
dc.relationRegulation of chromatin remodelling and gene expression during meiosis and Oocyte maturation
dc.relation0027030pt_PT
dc.relationWhen introns behave like exons: intron retention as a mechanism for dynamic regulation of gene expression
dc.relation.publisherversionhttps://rnajournal.cshlp.org/pt_PT
dc.subjectDrosophilapt_PT
dc.subjectGurkenpt_PT
dc.subjectDorsal-ventral patterningpt_PT
dc.subjectFemale fertilitypt_PT
dc.subjectSplicingpt_PT
dc.titleNineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterningpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstituto de Medicina Molecular
oaire.awardTitleREGULATION OF PRE-MRNA SPLICING DURING DROSOPHILA DEVELOPMENT
oaire.awardTitleRegulation of chromatin remodelling and gene expression during meiosis and Oocyte maturation
oaire.awardTitleWhen introns behave like exons: intron retention as a mechanism for dynamic regulation of gene expression
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBEX-BID%2F0395%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIA-BID%2F28441%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04773%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F50005%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F52428%2F2013/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F128283%2F2017/PT
oaire.citation.endPage1956pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage1935pt_PT
oaire.citation.titleRNApt_PT
oaire.citation.volume26pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream5876
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStreamOE
person.familyNameAscensão Ferreira
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person.givenNameMariana
person.givenNameCélia
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person.givenNameNUNO LUÍS
person.givenNameRui Goncalo
person.identifierhttps://scholar.google.com/citations?hl=pt-PT&user=NnJJEewAAAAJ
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