Publicação
Insight into pathogenomics and phylogeography of hypervirulent and highly-lethal Mycobacterium tuberculosis strain cluster
| dc.contributor.author | Mokrousov, Igor | |
| dc.contributor.author | Vyazovaya, Anna | |
| dc.contributor.author | Shitikov, Egor | |
| dc.contributor.author | Badleeva, Maria | |
| dc.contributor.author | Belopolskaya, Olesya | |
| dc.contributor.author | Bespiatykh, Dmitry | |
| dc.contributor.author | Gerasimova, Alena | |
| dc.contributor.author | Perdigão, João | |
| dc.date.accessioned | 2024-01-24T18:57:15Z | |
| dc.date.available | 2024-01-24T18:57:15Z | |
| dc.date.issued | 2023-06-23 | |
| dc.date.updated | 2023-12-29T12:13:18Z | |
| dc.description | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. | pt_PT |
| dc.description.abstract | Background The Mycobacterium tuberculosis Beijing genotype is globally spread lineage with important medical properties that however vary among its subtypes. M. tuberculosis Beijing 14717-15-cluster was recently discovered as both multidrug-resistant, hypervirulent, and highly-lethal strain circulating in the Far Eastern region of Russia. Here, we aimed to analyze its pathogenomic features and phylogeographic pattern. Results The study collection included M. tuberculosis DNA collected between 1996 and 2020 in different world regions. The bacterial DNA was subjected to genotyping and whole genome sequencing followed by bioinformatics and phylogenetic analysis. The PCR-based assay to detect specific SNPs of the Beijing 14717-15-cluster was developed and used for its screening in the global collections. Phylogenomic and phylogeographic analysis confirmed endemic prevalence of the Beijing 14717-15-cluster in the Asian part of Russia, and distant common ancestor with isolates from Korea (> 115 SNPs). The Beijing 14717-15-cluster isolates had two common resistance mutations RpsL Lys88Arg and KatG Ser315Thr and belonged to spoligotype SIT269. The Russian isolates of this cluster were from the Asian Russia while 4 isolates were from the Netherlands and Spain. The cluster-specific SNPs that significantly affect the protein function were identified in silico in genes within different categories (lipid metabolism, regulatory proteins, intermediary metabolism and respiration, PE/PPE, cell wall and cell processes). Conclusions We developed a simple method based on real-time PCR to detect clinically significant MDR and hypervirulent Beijing 14717-15-cluster. Most of the identified cluster-specific mutations were previously unreported and could potentially be associated with increased pathogenic properties of this hypervirulent M. tuberculosis strain. Further experimental study to assess the pathobiological role of these mutations is warranted. | pt_PT |
| dc.description.sponsorship | This study was supported by Russian Science Foundation (grant 19-14-00013). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Mokrousov I, Vyazovaya A, Shitikov E, Badleeva M, Belopolskaya O, Bespiatykh D, et al. Insight into pathogenomics and phylogeography of hypervirulent and highly-lethal Mycobacterium tuberculosis strain cluster. BMC Infect Dis [Internet]. 23 de junho de 2023;23(1):426. Disponível em: https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-023-08413-7 | pt_PT |
| dc.identifier.doi | 10.1186/s12879-023-08413-7 | pt_PT |
| dc.identifier.issn | 1471-2334 | |
| dc.identifier.slug | cv-prod-3440106 | |
| dc.identifier.uri | http://hdl.handle.net/10451/62085 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | BioMed Central | pt_PT |
| dc.relation.publisherversion | https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-023-08413-7 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Insight into pathogenomics and phylogeography of hypervirulent and highly-lethal Mycobacterium tuberculosis strain cluster | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 426 | pt_PT |
| oaire.citation.title | BMC Infectious Diseases | pt_PT |
| oaire.citation.volume | 23 | pt_PT |
| person.familyName | Lucas Mota Perdigão | |
| person.givenName | João Ruben | |
| person.identifier.ciencia-id | 991C-F26A-9843 | |
| person.identifier.orcid | 0000-0002-0339-1305 | |
| person.identifier.scopus-author-id | 24723211800 | |
| rcaap.cv.cienciaid | 991C-F26A-9843 | João Ruben Lucas Mota Perdigão | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 26de40fc-fc1d-4ff9-a242-3bb398e3ac1e | |
| relation.isAuthorOfPublication.latestForDiscovery | 26de40fc-fc1d-4ff9-a242-3bb398e3ac1e |