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Postembryonic establishment of megabase-scale gene silencing in nucleolar dominance

2007-11Journal article Open access
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dc.contributor.authorSilva, Manuela
dc.contributor.authorViegas, Wanda
dc.contributor.authorPontes, Olga
dc.contributor.authorLawrence, Richard
dc.contributor.authorPreuss, Sasha
dc.contributor.authorCosta-Nunes, Pedro
dc.contributor.authorEarley, Keith
dc.contributor.authorNeves, Nuno
dc.contributor.authorPikaard, Craig
dc.date.accessioned2010-10-26T15:12:14Z
dc.date.available2010-10-26T15:12:14Z
dc.date.issued2007-11
dc.description.abstractNucleolar dominance is an epigenetic phenomenon in plant and animal genetic hybrids that describes the expression of 45S ribosomal RNA genes (rRNA genes) inherited from only one progenitor due to the silencing of the other progenitor’s rRNA genes. rRNA genes are tandemly arrayed at nucleolus organizer regions (NORs) that span millions of basepairs, thus gene silencing in nucleolar dominance occurs on a scale second only to X-chromosome inactivation in female mammals. In Arabidopsis suecica, the allotetraploid hybrid of A. thaliana and A. arenosa, theA. thaliana –derived rRNA genes are subjected to nucleolar dominance and are silenced via repressive chromatin modifications. However, the developmental stage at which nucleolar dominance is established in A. suecica is currently unknown. We show that nucleolar dominance is not apparent in seedling cotyledons formed during embryogenesis but becomes progressively established during early postembryonic development in tissues derived from both the shoot and root apical meristems. The progressive silencing of A. thaliana rRNA genes correlates with the transition of A. thaliana NORs from a decondensed euchromatic state associated with histone H3 that is trimethylated on lysine 4 (H3K4me3) to a highly condensed heterochromatic state in which the NORs are associated with H3K9me2 and 5-methylcytosine-enriched chromocenters. In RNAi-lines in which the histone deacetylases HDA6 and HDT1 are knocked down, the developmentally regulated condensation and inactivation of A. thaliana NORs is disrupted. Collectively, these data demonstrate that HDA6 and HDT1 function in the postembryonic establishment of nucleolar dominance, a process which recurs in each generationpor
dc.identifier.citationPontes O, Lawrence RJ, Silva M, Preuss S, Costa-Nunes P, et al (2007) Postembryonic Establishment of Megabase-Scale Gene Silencing in Nucleolar Dominance. PLoS ONE 2(11): e1157por
dc.identifier.urihttp://hdl.handle.net/10400.5/2410
dc.language.isoengpor
dc.publisherPLoSpor
dc.relation.publisherversiondoi: 10.1371/journal.pone.0001157por
dc.subjectnucleolar dominancepor
dc.subjectgene silencingpor
dc.subjectepigeneticpor
dc.titlePostembryonic establishment of megabase-scale gene silencing in nucleolar dominancepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11por
oaire.citation.startPage1por
oaire.citation.titlePLoS ONEpor
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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