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Fase pré-analítica na morfologia parasitária
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| 4.55 MB | Adobe PDF |
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Abstract(s)
O presente relatório reporta as atividades laboratoriais acompanhadas durante o período de estágio no Laboratório de Análises Clínicas Dr. Joaquim Chaves, na clínica de Miraflores. O estágio encontra-se integrado na componente curricular do Mestrado em Análises Clínicas, da Faculdade de Farmácia da Universidade de Lisboa, compreendeu cerca de 1080 horas e teve como principais intuitos a aquisição de experiência profissional e a aplicação dos conhecimentos teóricos ministrados ao longo do mestrado.
O relatório sumariza as atividades acompanhadas nas seguintes valências: Bioquímica; Hematologia; Química Analítica; Imunologia; Radioimunoensaio; Microbiologia; Biologia Molecular e Genética. Aborda parâmetros analíticos, métodos laboratoriais e equipamentos, bem como a temática de higiene, saúde e segurança, as fases pré-analítica e pós-analítica e o controlo da qualidade realizado no Laboratório.
O estágio concretizado foi uma mais valia, dado que possibilitou consolidar e desenvolver os conhecimentos teóricos adquiridos no curso, contactar com metodologias e tecnologias avançadas e adquirir experiência profissional.
Este documento também desenvolve o seguinte tema: Fase Pré-Analítica na Morfologia Parasitária, em colaboração com o Instituto Nacional de Saúde Doutor Ricardo Jorge, I.P., no âmbito do Programa Nacional de Avaliação Externa da Qualidade.
O estudo inclui uma revisão bibliográfica sobre a qualidade no laboratório clínico e sobre os procedimentos da fase pré-analítica a realizar em produtos biológicos, para o diagnóstico microscópico de infeções parasitárias. Além disso, no âmbito deste estudo foram também desenvolvidas metodologias no Programa Nacional de Avaliação Externa da Qualidade de 2019, para aferir a fase pré-analítica na área da morfologia parasitária e o desempenho dos laboratórios participantes no Programa é apresentado e avaliado.
Faz parte dos objetivos deste trabalho, promover a consciencialização sobre a importância da fase pré-analítica na obtenção de resultados laboratoriais confiáveis, nomeadamente, na área da morfologia parasitária e pretende-se contribuir para a harmonização de procedimentos e de resultados entre laboratórios.
This report refers the laboratory activities that took place during the internship at the Laboratório de Análises Clínicas Dr. Joaquim Chaves (Miraflores). The internship is integrated in the study plan of the Master’s degree in Clinical Analysis of Faculdade de Farmácia da Universidade de Lisboa, it lasted about 1080 hours and its main purposes were the acquisition of professional experience and the application of theoretical knowledge acquired during the Master’s degree. The report summarizes the activities followed in diverse laboratory areas: Biochemistry; Haematology; Analytical Chemistry; Immunology; Radioimmunoassay; Microbiology; Molecular Biology and Genetics. It refers the analytical parameters, the laboratory methods and the equipment used, as well as hygiene, health and safety procedures, pre-analytical and post-analytical phases, and quality control performed at the Laboratory. This internship was an added value, because it allowed to consolidate and develop the theoretical knowledge obtained during the Master’s degree, contacting with advanced methodologies and technologies and acquire professional experience. This document also presents the following topic: Pre-Analytical Phase in Parasitic Morphology, in collaboration with the Instituto Nacional de Saúde Doutor Ricardo Jorge, under the National Program of External Quality Assessment. It was performed a bibliographic review about the quality in the clinical laboratory and about the pre-analytical procedures to be realized in biological specimens, for the microscopic diagnosis of parasitic infections. In addition, methodologies were also developed in the National Program of External Quality Assessment of 2019, to assess the pre-analytical phase in parasitic morphology area and the performance of the laboratories participating in the Program is presented and evaluated. This work aims to promote awareness about the importance of the pre-analytical phase in obtaining reliable laboratory results, namely in parasitic morphology area and it is intended to contribute for the harmonization of procedures and results between laboratories.
This report refers the laboratory activities that took place during the internship at the Laboratório de Análises Clínicas Dr. Joaquim Chaves (Miraflores). The internship is integrated in the study plan of the Master’s degree in Clinical Analysis of Faculdade de Farmácia da Universidade de Lisboa, it lasted about 1080 hours and its main purposes were the acquisition of professional experience and the application of theoretical knowledge acquired during the Master’s degree. The report summarizes the activities followed in diverse laboratory areas: Biochemistry; Haematology; Analytical Chemistry; Immunology; Radioimmunoassay; Microbiology; Molecular Biology and Genetics. It refers the analytical parameters, the laboratory methods and the equipment used, as well as hygiene, health and safety procedures, pre-analytical and post-analytical phases, and quality control performed at the Laboratory. This internship was an added value, because it allowed to consolidate and develop the theoretical knowledge obtained during the Master’s degree, contacting with advanced methodologies and technologies and acquire professional experience. This document also presents the following topic: Pre-Analytical Phase in Parasitic Morphology, in collaboration with the Instituto Nacional de Saúde Doutor Ricardo Jorge, under the National Program of External Quality Assessment. It was performed a bibliographic review about the quality in the clinical laboratory and about the pre-analytical procedures to be realized in biological specimens, for the microscopic diagnosis of parasitic infections. In addition, methodologies were also developed in the National Program of External Quality Assessment of 2019, to assess the pre-analytical phase in parasitic morphology area and the performance of the laboratories participating in the Program is presented and evaluated. This work aims to promote awareness about the importance of the pre-analytical phase in obtaining reliable laboratory results, namely in parasitic morphology area and it is intended to contribute for the harmonization of procedures and results between laboratories.
Description
Tese de mestrado, Análises Clínicas, 2020, Universidade de Lisboa, Faculdade de Farmácia.
Keywords
Pré-Analítica Bioquímica Hematologia Microbiologia Qualidade Teses de mestrado -2020