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Untargeted metabolomics reveals antidepressant effects in a marine photosynthetic organism: the diatom Phaeodactylum tricornutum as a case study

2022Journal article Open access
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dc.contributor.authorDuarte, Bernardo
dc.contributor.authorFeijão, Eduardo
dc.contributor.authorCruz de Carvalho, Ricardo
dc.contributor.authorDuarte, Irina A.
dc.contributor.authorMarques, Ana Patrícia
dc.contributor.authorMaia, Marisa
dc.contributor.authorHertzog, Jasmine
dc.contributor.authorMatos, Ana Rita
dc.contributor.authorCabrita, Maria Teresa
dc.contributor.authorCaçador, Isabel
dc.contributor.authorFigueiredo, Andreia
dc.contributor.authorSilva, Marta Sousa
dc.contributor.authorCordeiro, Carlos
dc.contributor.authorFonseca, Vanessa F.
dc.date.accessioned2023-01-27T10:30:59Z
dc.date.available2023-01-27T10:30:59Z
dc.date.issued2022
dc.description.abstractThe increased use of antidepressants, along with their increased occurrence in aquatic environments, is of concern for marine organisms. Although these pharmaceutical compounds have been shown to negatively affect marine diatoms, their mode of action in these non-target, single-cell phototrophic organisms is yet unknown. Using a Fourier-transform ion cyclotron-resonance mass spectrometer (FT-ICR-MS) we evaluated the effects of fluoxetine in the metabolomics of the model diatom Phaeodactylum tricornutum, as well as the potential use of the identified metabolites as exposure biomarkers. Diatom growth was severely impaired after fluoxetine exposure, particularly in the highest dose tested, along with a down-regulation of photosynthetic and carbohydrate metabolisms. Notably, several mechanisms that are normally down-regulated by fluoxetine in mammal organisms were also down-regulated in diatoms (e.g., glycerolipid metabolism, phosphatidylinositol signalling pathway, vitamin metabolism, terpenoid backbone biosynthesis and serotonin remobilization metabolism). Additionally, the present work also identified a set of potential biomarkers of fluoxetine exposure that were up-regulated with increasing fluoxetine exposure concentration and are of high metabolic significance following the disclosed mode of action, reinforcing the use of metabolomics approaches in ecotoxicology.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDuarte, B., Feijão, E., Cruz de Carvalho, R., Duarte, I. A., Marques, A. P., Maia, M., Hertzog, J., et al. (2022). Untargeted metabolomics reveals antidepressant effects in a marine photosynthetic organism: the diatom Phaeodactylum tricornutum as a case study. Biology, 11(12), 1770. http://dx.doi.org/10.3390/biology11121770pt_PT
dc.identifier.doi10.3390/biology11121770pt_PT
dc.identifier.issn2079-7737
dc.identifier.urihttp://hdl.handle.net/10451/56035
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0069/2020pt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationMarine and Environmental Sciences Centre
dc.relationCEECIND/02246/2017pt_PT
dc.relationBiosystems & Integrative Sciences Institute
dc.relationProject EU_FT-ICR_MSpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2079-7737/11/12/1770pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFluoxetinept_PT
dc.subjectMetabolomicspt_PT
dc.subjectEcotoxicologypt_PT
dc.subjectMarine diatomspt_PT
dc.titleUntargeted metabolomics reveals antidepressant effects in a marine photosynthetic organism: the diatom Phaeodactylum tricornutum as a case studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleBiosystems & Integrative Sciences Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04046%2F2019/PT
oaire.citation.issue12pt_PT
oaire.citation.startPage1770pt_PT
oaire.citation.titleBiologypt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCabrita
person.givenNameMaria Teresa
person.identifier349469
person.identifier.ciencia-id3E17-0125-F909
person.identifier.orcid0000-0002-4323-4945
person.identifier.ridB-5746-2014
person.identifier.scopus-author-id6602224932
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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