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Wolbachia screening in Aedes aegypti and Culex pipiens mosquitoes from Madeira Island, Portugal

2025Artigo científico Acesso aberto
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dc.contributor.authorFernandes, Rita
dc.contributor.authorMelo, Tiago
dc.contributor.authorZé-Zé, Líbia
dc.contributor.authorFreitas, Inês C.
dc.contributor.authorSilva, Manuel
dc.contributor.authorDias, Eva
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorGouveia, Bruna
dc.contributor.authorSeixas, Gonçalo
dc.contributor.authorOsório, Hugo
dc.date.accessioned2025-05-09T13:33:17Z
dc.date.available2025-05-09T13:33:17Z
dc.date.issued2025
dc.description© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractMosquito-borne diseases such as dengue and West Nile virus pose serious public health risks. On Madeira Island, the presence of the mosquito species Aedes aegypti (Linnaeus, 1762) and Culex pipiens (Linnaeus, 1758) raises concerns about local transmission. In this study, we tested 100 Ae. aegypti and 40 Cx. pipiens mosquitoes collected exclusively in the municipality of Funchal, Madeira Island, to assess the presence and diversity of Wolbachia, a naturally occurring bacterium known to reduce mosquitos' ability to transmit viruses. Molecular identification confirmed that all Cx. pipiens specimens belonged to the molestus biotype, with three individuals identified as hybrids between molestus and pipiens biotypes. This is the first evidence of such hybrids in Madeira. Wolbachia was not detected in any of the Ae. aegypti samples. In contrast, all Cx. pipiens mosquitoes were positive, showing a 100% prevalence. Genetic characterization placed these infections within the wPip clade, supergroup B, sequence type 9. These findings provide key baseline data to inform future mosquito control strategies on the island. As Ae. aegypti showed no natural Wolbachia infection, introducing Wolbachia-infected mosquitoes may be necessary to implement such biocontrol approaches in Madeira.pt_PT
dc.description.sponsorshipThis work was funded by the European Innovation Council and SMEs Executive Agency (EISMEA), project MOBVEC-Mobile Bio-Lab to support the first response in Arbovirus outbreaks, reference HORIZON-EIC-2022-PATHFINDEROPEN-01, with the grant agreement number 101099283; and PhD fellowships funded by Fundação para a Ciência e a Tecnologia (FCT), references: 2023.05286.BD and 2022.13476.BDANA. We also acknowledge the Interative Technologies Institute/Larsys, funded by FCT projects 10.54499/LA/P/0083/2020, 10.54499/UIDP/50009/2020, and 10.54499/UIDB/50009/2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInsects. 2025 Apr 15;16(4):418pt_PT
dc.identifier.doi10.3390/insects16040418pt_PT
dc.identifier.eissn2075-4450
dc.identifier.urihttp://hdl.handle.net/10400.5/100570
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation101099283pt_PT
dc.relation2023.05286.BDpt_PT
dc.relation2022.13476.BDANA.pt_PT
dc.relation10.54499/LA/P/0083/2020pt_PT
dc.relation10.54499/UIDP/50009/2020pt_PT
dc.relation10.54499/UIDB/50009/2020pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/journal/insectspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWolbachiapt_PT
dc.subjectBiological controlpt_PT
dc.subjectEndosymbiotic bacteriapt_PT
dc.subjectVector controlpt_PT
dc.titleWolbachia screening in Aedes aegypti and Culex pipiens mosquitoes from Madeira Island, Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue4pt_PT
oaire.citation.titleInsectspt_PT
oaire.citation.volume16pt_PT
person.familyNameLOPES DIAS
person.familyNameSantos
person.familyNameFigueira Ornelas de Gouveia
person.familyNameSeixas
person.familyNameCosta Osório
person.givenNameEVA MARIA
person.givenNameNuno
person.givenNameBruna Raquel
person.givenNameGonçalo
person.givenNameHugo
person.identifierF-9282-2014
person.identifier85792
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person.identifier.ciencia-idCD13-5E3A-A3C5
person.identifier.ciencia-idE212-42B7-575E
person.identifier.ciencia-idA118-C3E8-09C6
person.identifier.ciencia-id5C1B-84A4-ECC9
person.identifier.orcid0000-0003-0138-6999
person.identifier.orcid0000-0002-0580-0475
person.identifier.orcid0000-0001-7706-190X
person.identifier.orcid0000-0001-5745-2153
person.identifier.orcid0000-0001-6704-740X
person.identifier.ridN-7248-2013
person.identifier.ridJ-7802-2015
person.identifier.scopus-author-id55940818300
person.identifier.scopus-author-id55747279800
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication084d96dd-8ad9-4296-84bc-d8bb4c62af44
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