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Dithiothreitol revisited in red cells : a new head for an old hat
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Resumo(s)
In the present article the authors make an approach over the applications of dithiothreitol (DTT) in its different clinicallaboratory,
potential and up-to-date sources. Dithiothreitol is a chemical reagent with a wide actuation spectrum not only from
a laboratorial view but also from a therapeutic standpoint, more clinical and practical. DTT (i) is frequently used in a variety of
experiences that involve proteins or peptides, protecting sulfhydryl groups from oxidation and reducing disulfide bonds between
cysteines; (ii) is also used in the study of disulfide exchange reactions of protein disulfides; (iii) is able to keep glutathione in the
reduced state; (iv) acts as an “antidote” enabling the activity of detoxification systems; (v) participates in cellular mechanisms
such as vesiculation, cell morphology, signal transduction pathways (hormone-‘like’ role), etc.; (vi) can be used in the treatment
approach of diseases like cystinosis or medical conditions resulting from ion or metal toxicity. In erythrocytes, there’s literature
pointing that DTT may trigger changes on the normal discoid shape following metabolic depletion, and additionally modulate
the exovesiculation kinetics as demonstrated by us. The present article dissects in detail recent findings in our Unit concerning
the DTT influence on human erythrocytes.
Descrição
© 2010 – IOS Press and the authors. All rights reserved
Palavras-chave
Dithiothreitol Erythrocyte Redox status Thiols
Contexto Educativo
Citação
Clinical Hemorheology and Microcirculation 46 (2010) 51–56