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The NF-kappa B-signalling pathway in mare's endometrium infiltrated with the inflammatory cells
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Orientador(es)
Resumo(s)
ABSTRACT: Endometritis is an important issue decreasing mares' fertility. In the case of endo-metritis, both inflammatory cells infiltration and proinflammatory molecules produc-tion are regulated by various cellular and gene regulatory mechanisms, including the nuclear factor- κB (NF- κB)- dependent pathway. NF- κB- signalling pathway has been recently studied in the equine endometrium in the context of endometrosis. Thus, this study aimed to determine gene transcription of NF- κB subunits (RelA; NF- κB1; NF- κB2), proinflammatory molecules (M C P - 1 ; I L- 6) and hyaluronan synthases (HAS 1; HAS 2; HAS 3) in endometritis and compare them with the intensity and type of inflamma-tory cell infiltration. Endometrial samples, collected post- mortem from cyclic mares in oestrus or dioestrus, were classified histologically and examined using quantitative PCR. Transcription NF- κB subunits genes did not differ with either inflammatory in-tensity or type of inflammatory cell infiltration. Transcription of M C P - 1 and I L- 6 genes increased with the severity of inflammation, with the involvement of HAS 3 and HAS 2 genes, as opposed to HAS 1 genes. These proinflammatory molecules and hyaluronan synthases in the equine inflamed endometrium do not seem to be regulated by the NF- κB pathway. Hence, separate signalling pathways for the development and pro-gression of equine endometritis and endometrosis may be suggested
Descrição
Research Areas: Agriculture ; Reproductive Biology ; Veterinary Sciences
Palavras-chave
Endometritis HAS IL-6 Mare MCP-1 NF-kappa B
Contexto Educativo
Citação
Jasiński T, Zdrojkowski Ł, Kautz E, Juszczuk- Kubiak E, Ferreira-Dias G, Domino M. 2022. The NF- κB- signalling pathway in mare's endometrium infiltrated with the inflammatory cells. Reproduction in Domestic Animals, 57(6):598–610. DOI: https://doi.org/10.1111/rda.14099
Editora
Wiley