Repository logo
 
Publication

Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells

2023-02Journal article Open access
Simple item page
dc.contributor.authorAraújo, Diana
dc.contributor.authorRodrigues, Thomas
dc.contributor.authorRoma-Rodrigues, Catarina
dc.contributor.authorAlves, Vitor D.
dc.contributor.authorFernandes, Alexandra R.
dc.contributor.authorFreitas, Filomena
dc.date.accessioned2024-02-29T12:11:21Z
dc.date.available2024-02-29T12:11:21Z
dc.date.issued2023-02
dc.description.abstractChitin-glucan complex (CGC) hydrogels were fabricated by coagulation of the biopolymer from an aqueous alkaline solution, and their morphology, swelling behavior, mechanical, rheological, and biological properties were studied. In addition, their in vitro drug loading/release ability and permeation through mimic-skin artificial membranes (Strat-M) were assessed. The CGC hydrogels prepared from 4 and 6 wt% CGC suspensions (Na51*4 and Na51*6 hydrogels, respectively) had polymer contents of 2.40 0.15 and 3.09 0.22 wt%, respectively, and displayed a highly porous microstructure, characterized by compressive moduli of 39.36 and 47.30 kPa and storage moduli of 523.20 and 7012.25 Pa, respectively. Both hydrogels had a spontaneous and almost immediate swelling in aqueous media, and a high-water retention capacity (>80%), after 30 min incubation at 37 C. Nevertheless, the Na51*4 hydrogels had higher fatigue resistance and slightly higher-water retention capacity. These hydrogels were loaded with caffeine, ibuprofen, diclofenac, or salicylic acid, reaching entrapment efficiency values ranging between 13.11 0.49% for caffeine, and 15.15 1.54% for salicylic acid. Similar release profiles in PBS were observed for all tested APIs, comprising an initial fast release followed by a steady slower release. In vitro permeation experiments through Strat-M membranes using Franz diffusion cells showed considerably higher permeation fluxes for caffeine (33.09 g/cm2/h) and salicylic acid (19.53 g/cm2/h), compared to ibuprofen sodium and diclofenac sodium (4.26 and 0.44 g/cm2/h, respectively). Analysis in normal human dermal fibroblasts revealed that CGC hydrogels have no major effects on the viability, migration ability, and morphology of the cells. Given their demonstrated features, CGC hydrogels are very promising structures, displaying tunable physical properties, which support their future development into novel transdermal drug delivery platforms.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAraújo, D.; Rodrigues, T.; Roma-Rodrigues, C.; Alves, V.D.; Fernandes, A.R.; Freitas, F. Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells. Polymers 2023, 15, 791pt_PT
dc.identifier.doi10.3390/ polym15040791pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/30243
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.relationTransdermal delivery systems based on natural polymeric biomaterials
dc.relation.publisherversionhttps://www.mdpi.com/journal/polymerspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjecthydrogelspt_PT
dc.subjectchitin-glucan complexpt_PT
dc.subjectpolymer concentrationpt_PT
dc.subjectdrug deliverypt_PT
dc.subjectFranz diffusion cellpt_PT
dc.subjectpermeation studiespt_PT
dc.titleChitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardTitleTransdermal delivery systems based on natural polymeric biomaterials
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04129%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04129%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F140829%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F124612%2F2016/PT
oaire.citation.issue4pt_PT
oaire.citation.startPageArticle number 791pt_PT
oaire.citation.titlePolymerspt_PT
oaire.citation.volume15pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDelgado Alves
person.givenNameVitor
person.identifier1916677
person.identifier.ciencia-id2E1E-6918-4DB3
person.identifier.orcid0000-0002-4117-5582
person.identifier.scopus-author-id24536716000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8d1a8489-8789-4569-89e2-0bade23dd945
relation.isAuthorOfPublication.latestForDiscovery8d1a8489-8789-4569-89e2-0bade23dd945
relation.isProjectOfPublicationae38cc55-a200-4d0c-8c45-ec3fcb87b03e
relation.isProjectOfPublication5d9bae41-3108-4dac-b589-fbd8b25249a2
relation.isProjectOfPublicationf5a4cbcc-9670-4f3b-b01d-6c2b8778bbaa
relation.isProjectOfPublication242ffd71-e775-4d6a-8b04-1559d7f37ad1
relation.isProjectOfPublication68ef0aed-47b8-469e-819f-7175024862e9
relation.isProjectOfPublication08cf1ce2-e2e4-4e32-bbaa-71a53e3eea63
relation.isProjectOfPublication6f8f30e1-7382-4719-b24b-bb5442eb3840
relation.isProjectOfPublication.latestForDiscovery242ffd71-e775-4d6a-8b04-1559d7f37ad1

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ChitinGlucan-Complex-Hydrogels-PhysicalChemical-Characterization-Stability-In-Vitro-Drug-Permeation-and-Biological-Assessment-in-Primary-CellsPolymers.pdf
Size:
3.17 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISA - Artigos em Revistas Internacionais