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Virulence and resistance determinants of Klebsiella pneumoniae isolated from a Portuguese tertiary university hospital centre over a 31-year period

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dc.contributor.authorCaneiras, Catia
dc.contributor.authorLito, Luís
dc.contributor.authorMayoralas-Alises, Sagrario
dc.contributor.authorDíaz-Lobato, Salvador
dc.contributor.authorCristino, José Melo
dc.contributor.authorDuarte, Aida
dc.date.accessioned2019-06-18T16:20:25Z
dc.date.available2019-06-18T16:20:25Z
dc.date.issued2019
dc.description© 2018 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.pt_PT
dc.description.abstractIntroduction: The rapid and complex evolution of bacterial resistance mechanisms in Klebsiella pneumoniae producing extended-spectrum -lactamases and carbapenemases in Klebsiella pneumoniae is one of the most significant threats to public health. However, questions and controversies regarding the interactions between resistance and virulence in multidrug-resistant K. pneumoniae isolates remain unclear. Methods: A retrospective cohort study was performed with 100 K. pneumoniae isolates recovered from a tertiary care university hospital centre in Lisbon over a 31-year period. Resistance and virulence determinants were screened using molecular methods (PCR, M13-PCR and MLST). Results: The predominant virulence profile (fimH, mrkDv1, khe) was shared by all isolates, indicative of an important role of type 1 and 3 fimbrial adhesins and haemolysin, regardless of the type of -lactamase produced. However, accumulation of virulence factors was identified in KPC-3-producers, with a higher frequency (p < 0.05) of capsular serotype K2 and iucC aerobactin when compared with non-KPC-3 - lactamases or carbapenemases. Additionally, 9 different virulence profiles were found, indicating that the KPC-3 carbapenemase producers seem to adapt successfully to the host environment and maintain virulence via several pathways. Conclusion: This study describes an overlapping of multidrug-resistance and virulence determinants in ST-14K2 KPC-3 K. pneumoniae clinical isolates that may impose an additional challenge in the treatment of infections caused by this pathogen.pt_PT
dc.description.abstractIntroducción: La rápida y compleja evolución de los mecanismos de resistencia de Klebsiella pneumoniae productora de beta-lactamasas de espectro extendido y carbapenemasas en Klebsiella pneumoniae es una de las amenazas más importantes para la salud pública. Sin embargo, aun existe controversia sobre la interacción entre la resistencia y la virulencia en aislados de K. pneumoniae resistentes a múltiples antimicrobianos. Métodos: Se realizó un estudio de cohorte retrospectivo con 100 aislados de Klebsiella pneumoniae de un centro hospitalario universitario en Lisboa durante 31 anos. ˜ Los determinantes de la resistencia y virulencia se rastrearon utilizando métodos moleculares (PCR, M13-PCR y MLST). Resultados: Todos los aislados compartían un perfil de virulencia predominante (fimH, mrkDv1, khe), lo que indica un papel importante de las adhesinas fimbriales de tipo 1 y 3, y de la hemolisina, independientemente del tipo de -lactamasa producida. Sin embargo, la acumulación de factores de virulencia del serotipo capsular K2 y la aerobactina iucC se identificó con una mayor frecuencia en las cepas productoras de KPC-3 (p < 0,05) en comparación con las productoras de otras -lactamasas o carbapenemasas. Además, se encontraron 9 perfiles de virulencia diferentes, indicativos de que las cepas productoras de carbapenemasa KPC-3 parecen adaptarse con éxito al entorno y mantener la virulencia por varias vías. Conclusión: Este estudio describe la unión de resistencia a múltiples antimicrobianos junto con determinantes de virulencia en aislados clínicos de K. pneumoniae ST-14K2 KPC-3 lo que puede suponer un desafío adicional en el tratamiento de infecciones causadas por este patógeno.pt_PT
dc.description.sponsorshipThis work was supported by the Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEnferm Infecc Microbiol Clin. 2019;37(6):387-393pt_PT
dc.identifier.doi10.1016/j.eimc.2018.11.001pt_PT
dc.identifier.issn0213-005X
dc.identifier.urihttp://hdl.handle.net/10451/38738
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier Españapt_PT
dc.relation.publisherversionhttps://www.elsevier.es/es-revista-enfermedades-infecciosas-microbiologia-clinica-28pt_PT
dc.subjectKlebsiella pneumoniaept_PT
dc.subjectVirulencept_PT
dc.subjectBeta-lactamasespt_PT
dc.subjectBacterial infectionspt_PT
dc.subjectCross-infectionpt_PT
dc.titleVirulence and resistance determinants of Klebsiella pneumoniae isolated from a Portuguese tertiary university hospital centre over a 31-year periodpt_PT
dc.title.alternativeDeterminantes de virulencia y resistencia en Klebsiella pneumoniae aisladas en un centro hospitalario universitario en Portugal durante 31 anospt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage393pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage387pt_PT
oaire.citation.titleEnfermedades Infecciosas y Microbiología Clínicapt_PT
oaire.citation.volume37pt_PT
person.familyNameCaneiras
person.familyNameMelo Cristino
person.familyNameDuarte
person.givenNameCatia
person.givenNameJosé
person.givenNameAida
person.identifier2524696
person.identifier.ciencia-id5217-449F-3B4F
person.identifier.ciencia-id871E-6AD6-F37C
person.identifier.ciencia-idD517-F4DB-17C1
person.identifier.orcid0000-0002-3735-8554
person.identifier.orcid0000-0001-8643-1722
person.identifier.orcid0000-0002-0616-4650
person.identifier.ridH-3726-2013
person.identifier.ridM-5272-2013
person.identifier.scopus-author-id7004053640
person.identifier.scopus-author-id35495748300
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationacefbfd7-46d0-4429-80b1-ad159fe4ca95
relation.isAuthorOfPublicationf7620f51-a5ef-4e71-9cf5-3f34eb294a9d
relation.isAuthorOfPublication.latestForDiscovery67e72892-1980-4138-aeeb-244c4222d335

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