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Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium

2021-05-19Journal article Open access
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dc.contributor.authorAmaral, Ana
dc.contributor.authorFernandes, Carina
dc.contributor.authorSzóstek-Mioduchowska, Anna
dc.contributor.authorRebordão, Maria Rosa
dc.contributor.authorSkarzynski, Dariusz Jan
dc.contributor.authorFerreira-Dias, Graça
dc.date.accessioned2022-01-17T15:37:15Z
dc.date.available2022-01-17T15:37:15Z
dc.date.issued2021-05-19
dc.descriptionResearch Areas: Biochemistry & Molecular Biology ; Chemistrypt_PT
dc.description.abstractABSTRACT - Endometrosis is a reproductive pathology that is responsible for mare infertility. Our recent studies have focused on the involvement of neutrophil extracellular traps enzymes, such as elastase (ELA), in the development of equine endometrosis. Noscapine (NOSC) is an alkaloid derived from poppy opium with anticough, antistroke, anticancer, and antifibrotic properties. The present work investigates the putative inhibitory in vitro effect of NOSC on collagen type I alpha 2 chain (COL1A2) mRNA and COL1 protein relative abundance induced by ELA in endometrial explants of mares in the follicular or mid-luteal phases at 24 or 48 h of treatment. The COL1A2 mRNA was evaluated by qPCR and COL1 protein relative abundance by Western blot. In equine endometrial explants, ELA increased COL 1 expression, while NOSC inhibited it at both estrous cycle phases and treatment times. These findings contribute to the future development of new endometrosis treatment approaches. Noscapine could be a drug capable of preventing collagen synthesis in mare’s endometrium and facilitate the therapeutic approach.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmaral A, Fernandes C, Szóstek-Mioduchowska A, Rebordão MR, Skarzynski DJ, Ferreira-Dias G. 2021. Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine Endometrium. International Journal of Molecular Sciences. 22(10):5333. Doi:10.3390/ijms22105333pt_PT
dc.identifier.doi10.3390/ijms22105333pt_PT
dc.identifier.eissn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.5/23109
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPPN/BIL/2018/1/00250/U/0001pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosis
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/22/10/5333pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEquinept_PT
dc.subjectEndometrosispt_PT
dc.subjectFibrosispt_PT
dc.subjectElastasept_PT
dc.subjectNoscapinept_PT
dc.subjectInhibitionpt_PT
dc.subjectCollagenpt_PT
dc.titleNoscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometriumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-REP%2F4202%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F101058%2F2014/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volumev.22(10):5333pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNameAmaral
person.familyNameRebordão Cordeiro Simões Crisóstomo
person.familyNameLeitão Ferreira-Dias
person.givenNameAna
person.givenNameMaria Rosa
person.givenNameGraça Maria
person.identifier.ciencia-idEB18-9420-421B
person.identifier.ciencia-id9515-257A-B35D
person.identifier.ciencia-idF51C-7F45-C720
person.identifier.orcid0000-0002-0075-262X
person.identifier.orcid0000-0002-4389-445X
person.identifier.orcid0000-0003-0622-6513
person.identifier.scopus-author-id56394000500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationd1fd322a-70b1-4446-afe0-a5a4b9d1f5fa
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relation.isAuthorOfPublication.latestForDiscovery64ab1595-75c6-4b47-b196-ae1d494e6ca1
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