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Advisor(s)
Abstract(s)
ABSTRACT - Endometrosis is a reproductive pathology that is responsible for mare infertility. Our
recent studies have focused on the involvement of neutrophil extracellular traps enzymes, such as
elastase (ELA), in the development of equine endometrosis. Noscapine (NOSC) is an alkaloid derived
from poppy opium with anticough, antistroke, anticancer, and antifibrotic properties. The present
work investigates the putative inhibitory in vitro effect of NOSC on collagen type I alpha 2 chain
(COL1A2) mRNA and COL1 protein relative abundance induced by ELA in endometrial explants
of mares in the follicular or mid-luteal phases at 24 or 48 h of treatment. The COL1A2 mRNA was
evaluated by qPCR and COL1 protein relative abundance by Western blot. In equine endometrial
explants, ELA increased COL 1 expression, while NOSC inhibited it at both estrous cycle phases
and treatment times. These findings contribute to the future development of new endometrosis
treatment approaches. Noscapine could be a drug capable of preventing collagen synthesis in mare’s
endometrium and facilitate the therapeutic approach.
Description
Research Areas: Biochemistry & Molecular Biology ; Chemistry
Keywords
Equine Endometrosis Fibrosis Elastase Noscapine Inhibition Collagen
Pedagogical Context
Citation
Amaral A, Fernandes C, Szóstek-Mioduchowska A, Rebordão MR, Skarzynski DJ, Ferreira-Dias G. 2021. Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine Endometrium. International Journal of Molecular Sciences. 22(10):5333. Doi:10.3390/ijms22105333
Publisher
MDPI