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Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding

2022Journal article Open access
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dc.contributor.authorPinho, C.J.
dc.contributor.authorLopes, E.P.
dc.contributor.authorPaupério, J.
dc.contributor.authorGomes, I.
dc.contributor.authorRomeiras, Maria M.
dc.contributor.authorVasconcelos, R.
dc.date.accessioned2022-04-18T10:59:05Z
dc.date.available2022-04-18T10:59:05Z
dc.date.issued2022
dc.descriptionResearch Articlept_PT
dc.description.abstract1. DNA metabarcoding is widely used to characterize the diet of species, and it becomes very relevant for biodiversity conservation, allowing the understanding of trophic chains and the impact of invasive species. The need for cost-effective biodiversity monitoring methods fostered advances in this technique. One question that arises is which sample type provides a better diet representation. 2. Therefore, with this study, we intended to evaluate if there were differences in diet estimates according to the section of the gastrointestinal tract analysed and which section(s) provided the best diet representation. Additionally, we intended to infer the ecological/economic impacts of an invader as a model of the potential effects in an originally mammal-free ecosystem. 3. We examined the gut contents of the house mouse Mus musculus introduced to Cabo Verde, considering three sections: stomach, small intestine, and large intestine. We applied a DNA-metabarcoding approach using two genetic markers, one specific for plants and another for invertebrates. 4. We showed that this invader consumed 131 taxa (73 plants and 58 invertebrates). We obtained significant differences in the composition of two of the three sections, with a higher incidence of invertebrates in the stomach and plants in the intestines. This may be due to stomach inhibitors acting on plants and/or to faster absorption of soft-body invertebrates compared to the plant fibers in the intestines. We verified that the impact of this invader in the ecosystem is predominantly negative, as at least 50% of the ingested items were native, endemic, or economically important taxa, and only 19% of the diet items were exotics 5. Overall, results showed the need to analyse only two gastrointestinal tract sections to obtain robust diet data, increasing the cost-effectiveness of the method. Furthermore, by uncovering the native taxa most frequently preyed on by mice, this DNA-metabarcoding approach allowed us to evaluate efficiently which are at the highest riskpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEcology and Evolution. 2022;12:e8638pt_PT
dc.identifier.doihttps://doi.org/10.1002/ece3.8638pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/24094
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCabo Verde Islandspt_PT
dc.subjectdietpt_PT
dc.subjectgastrointestinal tractpt_PT
dc.subjecthouse mousept_PT
dc.subjectinvasive speciespt_PT
dc.subjectinvertebratespt_PT
dc.subjectnext-generation sequencingpt_PT
dc.subjectplantspt_PT
dc.titleTrust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcodingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04129%2F2020/PT
oaire.citation.titleEcology and Evolutionpt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRomeiras
person.givenNameMaria
person.identifier520836
person.identifier.ciencia-id6A1C-085E-9038
person.identifier.orcid0000-0002-9373-6302
person.identifier.ridJ-4552-2013
person.identifier.scopus-author-id15842082800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa66d151c-8bfa-4f32-ba8e-b20f9870ba24
relation.isAuthorOfPublication.latestForDiscoverya66d151c-8bfa-4f32-ba8e-b20f9870ba24
relation.isProjectOfPublication68ef0aed-47b8-469e-819f-7175024862e9
relation.isProjectOfPublication.latestForDiscovery68ef0aed-47b8-469e-819f-7175024862e9

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