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Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy

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dc.contributor.authorValentim Coelho, Cristina
dc.contributor.authorVaz, Fátima
dc.contributor.authorAntunes, Marilia
dc.contributor.authorNeves, Sofia
dc.contributor.authorMartins, Inês L.
dc.contributor.authorOsório, Hugo
dc.contributor.authorFeliciano, Amélia
dc.contributor.authorPinto, Paula
dc.contributor.authorBárbara, Cristina
dc.contributor.authorPenque, Deborah
dc.date.accessioned2020-12-10T16:38:08Z
dc.date.available2020-12-10T16:38:08Z
dc.date.issued2020
dc.description© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractIn this study, we examined the effect of six months of positive airway pressure (PAP) therapy on Obstructive Sleep Apnea (OSA) red blood cell (RBC) proteome by two dimensional difference gel electrophoresis (2D-DIGE) - based proteomics followed by Western blotting (WB) validation. The discovered dysregulated proteins/proteoforms are associated with cell death, H2O2 catabolic/metabolic process, stress response, and protein oligomerization. Validation by nonreducing WB was performed for peroxiredoxin-2 (PRDX2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by using antibodies against the sulfinylated/sulfonylated cysteine of these proteins to better evaluate their redox-oligomeric states under OSA and/or in response to PAP therapy. The results indicated that the redox-oligomeric state of GAPDH and PRDX2 involving overoxidation by sulfinic/sulfonic acids were differentially modulated in OSA RBC, which might be compromising RBC homeostasis. PAP therapy by restoring this modulation induced a higher oligomerization of overoxidized GAPDH and PRDX2 in some patients that could be associated with eryptosis and the chaperone "gain" of function, respectively. This varied response following PAP may result from the complex interplay between OSA and OSA metabolic comorbidity. Hence, information on the redox status of PRDX2 and GAPDH in RBC will help to better recognize OSA subtypes and predict the therapeutic response in these patients. GAPDH monomer combined with body mass index (BMI) and PRDX2 S-S dimer combined with homeostatic model assessment for insulin resistance (HOMA-IR) showed to be very promising biomarkers to predict OSA and OSA severity, respectively.pt_PT
dc.description.sponsorshipProject partially supported by Harvard Medical School-Portugal Program (HMSPICJ/0022/2011), ToxOmics - Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013), Fundação para a Ciência e a Tecnologia (FCT)/Poly-Annual Funding Program and FEDER/Saúde XXI Program, Portugal, RNEM- National Mass Spectrometry Networking—FCT Strategic Infrastructure and PhD fellowship, FCT-SFRH/BD/133511/2017, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntioxidants 2020, 9, 1184.pt_PT
dc.identifier.doi10.3390/antiox9121184pt_PT
dc.identifier.issn2076-3921
dc.identifier.urihttp://hdl.handle.net/10451/45242
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationProteome Profiling in Obstructive Sleep Apnea Severity and Treatment Response Towards Early Diagnosis and Prognosis Prediction
dc.relation.publisherversionhttps://www.mdpi.com/journal/antioxidantspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectObstructive sleep apnea (OSA)pt_PT
dc.subjectPositive airway pressure (PAP)pt_PT
dc.subjectPeroxiredoxin-2 (PRDX2)pt_PT
dc.subjectGlyceraldehyde-3-phosphate dehydrogenase (GAPDH)pt_PT
dc.subjectCys-sulfinylation/Cys-sulfonylationpt_PT
dc.subjectProteomics-biomarkerspt_PT
dc.titleRedox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUID/BIM/00009/2013
oaire.awardNumberSFRH/BD/133511/2017
oaire.awardTitleProteome Profiling in Obstructive Sleep Apnea Severity and Treatment Response Towards Early Diagnosis and Prognosis Prediction
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F133511%2F2017/PT
oaire.citation.issue12pt_PT
oaire.citation.startPage1184pt_PT
oaire.citation.titleAntioxidantspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream5876
oaire.fundingStreamOE
person.familyNamede Sousa Antunes
person.familyNameCosta Osório
person.familyNamePinto
person.familyNameBárbara
person.givenNameMarilia Cristina
person.givenNameHugo
person.givenNamePaula Maria Gonçalves
person.givenNameCristina
person.identifierG-8864-2015
person.identifier85792
person.identifier1330713
person.identifier.ciencia-id1A16-4A6A-A8F9
person.identifier.ciencia-id5C1B-84A4-ECC9
person.identifier.ciencia-idF01D-6AFB-DFFD
person.identifier.ciencia-id171F-DE21-B23B
person.identifier.orcid0000-0002-1257-2829
person.identifier.orcid0000-0001-6704-740X
person.identifier.orcid0000-0003-2301-2752
person.identifier.orcid0000-0003-0915-4105
person.identifier.ridJ-7802-2015
person.identifier.scopus-author-id16300632900
person.identifier.scopus-author-id55747279800
person.identifier.scopus-author-id24074744900
person.identifier.scopus-author-id6701574502
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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