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Resumo(s)
A produção de refrigerantes de qualidade exige matérias primas e subsidiárias excelentes e a garantia da não contaminação durante o fabrico. Neste contexto, as . operações de sanificação são um factor essencial. Com o objectivo de optimizar o programa de sanificação para duas linhas de produção de refrigerantes em lata, realizaram-se análises microbiológicas, a fim de avaliar a eficácia, em diferentes pontos das linhas. A morosidade das respostas obtidas pelos métodos microbiologicos clássicos apenas permite uma atitude preventiva a posteriori. Tendo em vista ultrapassar esta limitação realizaram-se estudos para aplicação da bioluminescência ao controlo desses processos de sanificação, como base de um método pró-activo. Os resultados obtidos permitiram detectar deficiências no programa de sanificação e estabelecer pontos críticos de controlo. A técnica da bioluminescência, não tem sensibilidade para as especificações exigidas por esta indústria e os resultados dependem da forma como o protocolo é executado. Contudo, esta técnica é util para detectar deficiências das lavagens. Ambas as técnicas têm inconvenientes. A existência de programas de sanificação para cada produto, equipamento e indústria, são a chave para a garantia da qualidade higio-sanitária de uma produção. Os ensaios in vitro para determinar a eficácia do detergente clorado, mostraram que, concentrações entre os 8 a 10 ppm de cloro activo, aplicadas durante 4 horas, reduzem 3 a 5 ciclos logarítmicos a população inicial, na maioria dos microrganismos estudados. O Penicillium nota/um foi o mais resistente e apenas se registou uma redução de 2 ciclos.
The production of good quality soft drinks demands good raw and subsidiary materiais and the guarantee of no microbiological contamination during the process. Plant and equipment sanitation is therefore an essencial factor. With the aim of optimizing the sanitation program for two soft drinks can lines, microbiological analysis were conducted, to evaluate the sanitation effíciency in different points of the fines. The classical microbiological methods only enable a preventive attitude a posteriori, due to the incubation delay. Trying to overtake this limitation, the application of bioluminescence on the control of the sanitation processes was studied, based in a pro-active method. The results obtained enabled to detect defficiencies in the sanitation program and to estabfish crifical control poirits. The sensibility of the bioluminescence technique is lower than the one required by the stringent specifications of the soft drinks industry. Also, the results are dependent on the way the protocol is conducted. However, this technique is useful to detect defficiencies in the cleaning process. Both techniques present inconvenients. The existence of specific sanitation programs for each product, equipment and industry, is the key for the guarantee of a production sanitary quality. The essays in vitro to evaluate the effíciency of the chlorine saffitizer, showed that a concentrafion between 8 and 10 ppm of active chlorine, acting during 4 hours, reduces 3 to 5 logaritmic cycles the initial populafion, for the majority of the microrganisms considered in the study. The Penicillium notatum was the most resistant and only a reduction of 2 cycles was observed.
The production of good quality soft drinks demands good raw and subsidiary materiais and the guarantee of no microbiological contamination during the process. Plant and equipment sanitation is therefore an essencial factor. With the aim of optimizing the sanitation program for two soft drinks can lines, microbiological analysis were conducted, to evaluate the sanitation effíciency in different points of the fines. The classical microbiological methods only enable a preventive attitude a posteriori, due to the incubation delay. Trying to overtake this limitation, the application of bioluminescence on the control of the sanitation processes was studied, based in a pro-active method. The results obtained enabled to detect defficiencies in the sanitation program and to estabfish crifical control poirits. The sensibility of the bioluminescence technique is lower than the one required by the stringent specifications of the soft drinks industry. Also, the results are dependent on the way the protocol is conducted. However, this technique is useful to detect defficiencies in the cleaning process. Both techniques present inconvenients. The existence of specific sanitation programs for each product, equipment and industry, is the key for the guarantee of a production sanitary quality. The essays in vitro to evaluate the effíciency of the chlorine saffitizer, showed that a concentrafion between 8 and 10 ppm of active chlorine, acting during 4 hours, reduces 3 to 5 logaritmic cycles the initial populafion, for the majority of the microrganisms considered in the study. The Penicillium notatum was the most resistant and only a reduction of 2 cycles was observed.
Descrição
Mestrado em Ciência e Tecnologia de Alimentos
Palavras-chave
Desinfecção Sanificação Indústria Refrigerantes Microbiologia e Bioluminescência Desinfection Sanitation Industry Soft drinks Microbiology and Biohuninescence
Contexto Educativo
Citação
Mestre, Maria de Jesus Urbano 1995). “Eficácia dos programas de sanificação na indústria de refrigerantes : modelo microbiológico ”. Dissertação de Mestrado. Universidade Técnica de Lisboa
Editora
Universidade Técnica de Lisboa
