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Competition for endothelial cell polarity drives vascular morphogenesis in the mouse retina

2022Journal article Open access
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dc.contributor.authorBarbacena, Pedro
dc.contributor.authorDominguez-Cejudo, Maria Angeles
dc.contributor.authorFonseca, Catarina
dc.contributor.authorGómez-González, Manuel
dc.contributor.authorFaure, Laura M.
dc.contributor.authorZarkada, Georgia
dc.contributor.authorPena, Andreia
dc.contributor.authorPezzarossa, Anna
dc.contributor.authorRamalho, Daniela
dc.contributor.authorGiarratano, Ylenia
dc.contributor.authorOuarné, Marie
dc.contributor.authorBarata, David
dc.contributor.authorFortunato, Isabela C.
dc.contributor.authorHenao Mišíková, Lenka
dc.contributor.authorMauldin, Ian
dc.contributor.authorCarvalho, Yulia
dc.contributor.authorTrepat, Xavier
dc.contributor.authorRoca-Cusachs, Pere
dc.contributor.authorEichmann, Anne
dc.contributor.authorBernabeu, Miguel O.
dc.contributor.authorFranco, Claudio
dc.date.accessioned2022-10-21T15:26:10Z
dc.date.available2022-10-21T15:26:10Z
dc.date.issued2022
dc.description© 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).pt_PT
dc.description.abstractBlood-vessel formation generates unique vascular patterns in each individual. The principles governing the apparent stochasticity of this process remain to be elucidated. Using mathematical methods, we find that the transition between two fundamental vascular morphogenetic programs-sprouting angiogenesis and vascular remodeling-is established by a shift of collective front-to-rear polarity of endothelial cells in the mouse retina. We demonstrate that the competition between biochemical (VEGFA) and mechanical (blood-flow-induced shear stress) cues controls this collective polarity shift. Shear stress increases tension at focal adhesions overriding VEGFA-driven collective polarization, which relies on tension at adherens junctions. We propose that vascular morphogenetic cues compete to regulate individual cell polarity and migration through tension shifts that translates into tissue-level emergent behaviors, ultimately leading to uniquely organized vascular patterns.pt_PT
dc.description.sponsorshipFunding: European Research Council: C.A.F. (679368); X.T. (883739). European Commission: C.A.F. and M.O.B. (801423); X.T. and P.R.-C. (731957). H2020-MSCA-PF grants to M.G.-G. (797621) and M.O. (842498). Fondation LeDucq: C.A.F., A.E., and M.O.B. (17CVD03). EPSRC: M.O.B. (EP/T008806/1; EP/R029598/1). Fundação para a Ciência e Tecnologia: C.A.F. (PTDC/MED-PAT/31639/2017; PTDC/BIA-CEL/32180/2017; CEECIND/04251/2017). Spanish Ministry of Science and Innovation: P.R.-C. (PID2019-110298GB-I00); X.T. (PGC2018-099645-B-I00). Generalitat de Catalunya: X.T. and P.R.-C. (2017-SGR-1602). La Caixa Foundation: X.T. and P.R.-C. (LCF/PR/HR20/52400004). Fundació la Marató de TV3: X.T. (201903-30-31-32). EMBO: L.M.F. (ALTF 2-2018)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDev Cell. 2022 Oct 10;57(19):2321-2333.e9pt_PT
dc.identifier.doi10.1016/j.devcel.2022.09.002pt_PT
dc.identifier.eissn1878-1551
dc.identifier.issn1534-5807
dc.identifier.urihttp://hdl.handle.net/10451/54853
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPRINCIPLES OF AXIAL POLARITY-DRIVEN VASCULAR PATTERNING
dc.relationEngineering epithelial shape and mechanics: from synthetic morphogenesis to biohybrid devices
dc.relationBuilding a 3D innervated and irrigated muscle on a chip.
dc.relationMechanical control of biological function
dc.relationProbing and controlling the three-dimensional organoid mechanobiology
dc.relationAdaptive and maladaptive endothelial cell dynamics during blood flow-driven vascular patterning
dc.relationDeregulated Endothelial Blood Flow Response as a cause of Diabetic Retinopathy
dc.relationIdentify the mechanisms of endothelial tip cell invasive behavior in order to inhibit physiological and pathological sprouting angiogenesis
dc.relationNot Available
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/developmental-cellpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAngiogenesispt_PT
dc.subjectCell polaritypt_PT
dc.subjectMechanobiologypt_PT
dc.subjectMorphogenesispt_PT
dc.subjectShear stresspt_PT
dc.titleCompetition for endothelial cell polarity drives vascular morphogenesis in the mouse retinapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePRINCIPLES OF AXIAL POLARITY-DRIVEN VASCULAR PATTERNING
oaire.awardTitleEngineering epithelial shape and mechanics: from synthetic morphogenesis to biohybrid devices
oaire.awardTitleBuilding a 3D innervated and irrigated muscle on a chip.
oaire.awardTitleMechanical control of biological function
oaire.awardTitleProbing and controlling the three-dimensional organoid mechanobiology
oaire.awardTitleAdaptive and maladaptive endothelial cell dynamics during blood flow-driven vascular patterning
oaire.awardTitleDeregulated Endothelial Blood Flow Response as a cause of Diabetic Retinopathy
oaire.awardTitleIdentify the mechanisms of endothelial tip cell invasive behavior in order to inhibit physiological and pathological sprouting angiogenesis
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/679368/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/883739/EU
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMED-PAT%2F31639%2F2017/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F04251%2F2017%2FCP1396%2FCT0010/PT
oaire.citation.endPage2333.e9pt_PT
oaire.citation.issue19pt_PT
oaire.citation.startPage2321pt_PT
oaire.citation.titleDevelopmental Cellpt_PT
oaire.citation.volume57pt_PT
oaire.fundingStreamH2020
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person.familyNameBarbacena
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person.familyNameHenao Mišíková
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person.givenNameMaria Angeles
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person.givenNameDaniela Maria
person.givenNameMarie
person.givenNameDavid
person.givenNameIsabela Corina
person.givenNameLenka
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project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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