Publicação
Identification of critical points in colloidal delivery systems for intravenous administration and intracellular delivery of nucleic acids as therapeutic agents
| dc.contributor.advisor | Videira, Mafalda | por |
| dc.contributor.advisor | Gaspar, Rogério | por |
| dc.contributor.author | Mouro, Andreia Alexandra Dias | |
| dc.date.accessioned | 2014-05-12T15:31:13Z | |
| dc.date.available | 2014-05-12T15:31:13Z | |
| dc.date.issued | 2012 | |
| dc.description | Tese de mestrado, Farmacotecnia Avançada (Tecnologia Farmacêutica), Universidade de Lisboa, Faculdade de Farmácia, 2012 | por |
| dc.description.abstract | The RNA-mediated gene-silencing technology, carried out by small interfering RNAs (siRNAs), has attracted a great deal of attention as novel promising therapeutic strategy in oncology. One of the common themes emerging from the studies on cell-specific delivery of siRNA is the need for optimizing the intracellular trafficking of the siRNA to elicit a silencing response. Polymer nanoparticles have become recognized as an efficiency strategy for oligonucleotide delivery to a specific cell population. Among these carriers, PLGA-co-PEG nanoparticles have attracted much attention since they are assumed to meet the criteria required for successful siRNA delivery: they are sufficiently small for efficient tissue penetration and cellular uptake and offer physical protection against RNase activity as well as a favorable colloidal stability. In this study the ability of a polymeric micelle based system for the targeting and delivery of a siRNA to breast cancer cells was proved using as model the siRNA against the Green Fluorescence Protein (GFP). The efficiencies observed during in vitro studies with a MDA-MB-436/GFP cell line confirmed the potential of this new delivery system but it needs further investigation. | por |
| dc.identifier.uri | http://hdl.handle.net/10451/11011 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.subject | Polymeric micelles | por |
| dc.subject | siRNA | por |
| dc.subject | PLGA-co-PEG | por |
| dc.subject | Green Fluorescence Protein (GFP) | por |
| dc.subject | Teses de mestrado - 2012 | por |
| dc.title | Identification of critical points in colloidal delivery systems for intravenous administration and intracellular delivery of nucleic acids as therapeutic agents | por |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | por |
| rcaap.type | masterThesis | por |