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Identification of critical points in colloidal delivery systems for intravenous administration and intracellular delivery of nucleic acids as therapeutic agents
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Resumo(s)
The RNA-mediated gene-silencing technology, carried out by small interfering RNAs (siRNAs), has attracted a great deal of attention as novel promising therapeutic strategy in oncology.
One of the common themes emerging from the studies on cell-specific delivery of siRNA is the need for optimizing the intracellular trafficking of the siRNA to elicit a silencing response. Polymer nanoparticles have become recognized as an efficiency strategy for oligonucleotide delivery to a specific cell population.
Among these carriers, PLGA-co-PEG nanoparticles have attracted much attention since they are assumed to meet the criteria required for successful siRNA delivery: they are sufficiently small for efficient tissue penetration and cellular uptake and offer physical protection against RNase activity as well as a favorable colloidal stability.
In this study the ability of a polymeric micelle based system for the targeting and delivery of a siRNA to breast cancer cells was proved using as model the siRNA against the Green Fluorescence Protein (GFP). The efficiencies observed during in vitro studies with a MDA-MB-436/GFP cell line confirmed the potential of this new delivery system but it needs further investigation.
Descrição
Tese de mestrado, Farmacotecnia Avançada (Tecnologia Farmacêutica), Universidade de Lisboa, Faculdade de Farmácia, 2012
Palavras-chave
Polymeric micelles siRNA PLGA-co-PEG Green Fluorescence Protein (GFP) Teses de mestrado - 2012