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Inflammatory response in rats upon intratracheal instillation of sterigmatocystin and 5-methoxysterigamtocystin
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As micotoxinas são metabolitos secundários tóxicos produzidos por fungos filamentosos. Podem ser ingeridas, inaladas ou absorvidas, através da pele.
Estas substâncias são facilmente encontradas em ambientes fechados, através de esporos que circulam pelo ar, na qual pode causar vários efeitos sérios, como cronicidade nos diversos sistemas do animal.
A esterigmatocistina (STC) é uma das micotoxinas utilizadas em estudo, e em 1954 foi isolada pela primeira vez da espécie Aspergillus versicolor e é um precursor da aflatoxina B1. Verificou-se que a micotoxina 5-metoxi-esterigmatocistina (5-Met-STC) é derivada da STC e, diferem a nível estrutural por um grupo metoxi na posição 11, mais precisamente.
A STC e a aflatoxina B1 (AFB1) partilham algumas características como a genotoxicidade e a carcinogenicidade, considerando assim estas duas estruturas similares.
A maioria dos estudos abordam a toxicidade, mas a nível de ingestão, por isso neste trabalho estudou-se outro método, a instilação intra-traqueal. Assim, as citocinas Interleucina 6 (IL-6), Fator necrose tumoral alfa (TNF-α) e Proteína-1 alfa inflamatória macrofágica (MIP-1α) foram utilizadas para verificar a resposta inflamatória que desencadeou nos ratinhos em estudo.
As diferenças nos resultados entre as micotoxinas podem ser devidas ao grupo metoxi que o 5-Met-STC contém a mais.
Mycotoxins are secondary metabolites for filamentous fungi, which become toxic when ingested, inhaled or absorbed, through the skin. It is easily found in closed environments, through the spores that walk in the air, which can cause very serious effects, such as chronicity in several animal systems. Sterigmatocystin (STC) is one of the mycotoxins used in the study, and in 1954 it was isolated for the first time from the species Aspergillus versicolor and is a precursor to aflatoxin B1 (AFB1). Mycotoxin 5-Methoxy-Sterigmatocystin (5-Met-STC) has been found to be derived from STC and differ structurally by a methoxy group at position 11 more precisely. STC and AFB1 share some characteristics such as genotoxicity and carcinogenicity, thus considering these two structures as similar. Most studies address toxicity but at the level of ingestion, so in this work another aspect was studied, the intratracheal instillation method. Thus, the cytokine Interleukin-6 human (IL-6), Tumor Necrosis Factor-alpha (TNF-α) and Macrophage Inflammatory Protein-1 Alpha (MIP-1α) were used to ascertain the inflammatory response that triggered the rats under study. Differences in results between mycotoxins may be due to the methoxy group that 5-Met-STC contains the most.
Mycotoxins are secondary metabolites for filamentous fungi, which become toxic when ingested, inhaled or absorbed, through the skin. It is easily found in closed environments, through the spores that walk in the air, which can cause very serious effects, such as chronicity in several animal systems. Sterigmatocystin (STC) is one of the mycotoxins used in the study, and in 1954 it was isolated for the first time from the species Aspergillus versicolor and is a precursor to aflatoxin B1 (AFB1). Mycotoxin 5-Methoxy-Sterigmatocystin (5-Met-STC) has been found to be derived from STC and differ structurally by a methoxy group at position 11 more precisely. STC and AFB1 share some characteristics such as genotoxicity and carcinogenicity, thus considering these two structures as similar. Most studies address toxicity but at the level of ingestion, so in this work another aspect was studied, the intratracheal instillation method. Thus, the cytokine Interleukin-6 human (IL-6), Tumor Necrosis Factor-alpha (TNF-α) and Macrophage Inflammatory Protein-1 Alpha (MIP-1α) were used to ascertain the inflammatory response that triggered the rats under study. Differences in results between mycotoxins may be due to the methoxy group that 5-Met-STC contains the most.
Descrição
Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, 2020, Universidade de Lisboa, Faculdade de Farmácia.
Palavras-chave
STC 5-Met-STC IL-6 TNF-α MIP-1α Mestrado integrado - 2020