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A metabarcoding tool to detect predation of the honeybee Apis mellifera and other wild insects by the invasive Vespa velutina

2021-06Journal article Open access
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dc.contributor.authorVerdasca, Maria João
dc.contributor.authorGodinho, Raquel
dc.contributor.authorRocha, Rita Gomes
dc.contributor.authorPortocarrero, Marco
dc.contributor.authorGigante Carvalheiro, Luísa
dc.contributor.authorSampaio e rebelo, Rui
dc.contributor.authorRebelo, Hugo
dc.date.accessioned2021-11-17T19:58:11Z
dc.date.available2022-06-04T00:30:29Z
dc.date.issued2021-06
dc.description.abstractThe invasive Vespa velutina has been widely referred as an effective predator of honeybees. Despite the potential risk to pollination services provision and honey production, there is no accurate quantification and assessment of its real consequences for honeybees. To date, the identification of the honeybee and other insects in the diet of V. velutina has been investigated by direct observation of adult foraging or examination of food pellets. To overcome these limitations, in this study we used a DNA metabarcoding approach to evaluate the usefulness of different types of sample (jaws and stomachs collected from workers and larval faecal pellets taken from the hornet comb) to investigate the predation of V. velutina upon honeybees, and potentially on other insects. Honeybee DNA was identified in all types of samples, but larval faecal pellets retrieved the higher number of reads of honeybee DNA and the largest diversity at all taxonomic levels. Over all samples we could identify 4 orders, 9 families, 6 genera and 1 species of prey. We estimate that collecting 6 workers is sufficient to identify honeybee predation by a colony using worker’s jaws. Stomachs were the least useful sample type to detect honeybee DNA. The presence of honeybee DNA in all analysed colonies irrespective of collection site, and the variety of insect orders detected in the diet support current concerns over the acknowledged negative impact of V. velutina on managed honeybees and its potential threat to pollination services provision.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVerdasca, M.J., Godinho, R., Rocha, R.G. et al. A metabarcoding tool to detect predation of the honeybee Apis mellifera and other wild insects by the invasive Vespa velutina. J Pest Sci (2021). https://doi.org/10.1007/s10340-021-01401-3pt_PT
dc.identifier.doi10.1007/s10340-021-01401-3pt_PT
dc.identifier.issn1612-4766
dc.identifier.urihttp://hdl.handle.net/10451/50138
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationFCT project UIDB/00329/2020pt_PT
dc.relationFCT PD/BD/128351/2017pt_PT
dc.relationFCT contract DL/2016pt_PT
dc.relationFCT CEECIND/01087/2018pt_PT
dc.relationFCT LISBOA-01-0145-FEDER-028360/EUCLIPOpt_PT
dc.relationPromoting the resilience of ecosystem services to climate change: a case study with pine plantations, pine processionary moth and bats
dc.relationBrazilian National Council for Scientific and Technological Development PQ 305157/2018-3pt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10340-021-01401-3pt_PT
dc.subjectApis melliferapt_PT
dc.subjectdiet analysispt_PT
dc.subjectDNA metabarcodingpt_PT
dc.subjectinvasive speciespt_PT
dc.subjecttaxon-specific primerspt_PT
dc.subjectVespa velutinapt_PT
dc.titleA metabarcoding tool to detect predation of the honeybee Apis mellifera and other wild insects by the invasive Vespa velutinapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePromoting the resilience of ecosystem services to climate change: a case study with pine plantations, pine processionary moth and bats
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIA-ECO%2F31731%2F2017/PT
oaire.citation.titleJournal of Pest Sciencept_PT
oaire.fundingStream9471 - RIDTI
person.familyNameCaldeira Verdasca
person.familyNameGigante Carvalheiro
person.familyNameBorges Sampaio e Rebelo
person.givenNameMaria João
person.givenNameLuísa
person.givenNameRui Miguel
person.identifier.ciencia-id091F-CDD7-74DA
person.identifier.ciencia-idC816-BF92-42C5
person.identifier.ciencia-id651A-9349-61EF
person.identifier.orcid0000-0002-1595-0338
person.identifier.orcid0000-0001-7655-979X
person.identifier.orcid0000-0003-2544-1470
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctO tipo de licença (Standard Licence) envolvido com a editora Springer Nature para esta revista, não envolvendo pagamento (APC), apenas permite acesso em repositório público do formato 'aceite' (i.e não editado e paginado pela editora) após período de embargo de 12 meses após a data da sua publicação final.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication37b8cd09-c1e7-42dc-9a74-aa3f880a63b2
relation.isAuthorOfPublication621a851c-d356-4e35-857d-e6ffac529a13
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