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The Free-Living Stage Growth Conditions of the Endophytic Fungus Serendipita indica May Regulate Its Potential as Plant Growth Promoting Microbe

2020-09Artigo científico Acesso aberto
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dc.contributor.authorDias, Teresa
dc.contributor.authorPimentel, Vívian
dc.contributor.authorCogo, Antônio Jesus Dorighetto
dc.contributor.authorCosta, Raquel
dc.contributor.authorBertolazi, Amanda Azevedo
dc.contributor.authorMiranda, Camila
dc.contributor.authorde Souza, Sávio Bastos
dc.contributor.authorMelo, Juliana
dc.contributor.authorCarolino, Manuela
dc.contributor.authorVarma, Ajit
dc.contributor.authorEutrópio, Frederico
dc.contributor.authorOlivares, Fábio Lopes
dc.contributor.authorRamos, Alessandro Coutinho
dc.contributor.authorCruz, Cristina
dc.date.accessioned2021-09-27T13:00:57Z
dc.date.available2021-09-27T13:00:57Z
dc.date.issued2020-09
dc.description.abstractSerendipita indica (former Piriformospora indica) is a non-obligate endophytic fungus and generally a plant growth and defence promoter with high potential to be used in agriculture. However, S. indica may switch from biotrophy to saprotrophy losing its plant growth promoting traits. Our aim was to understand if the free-living stage growth conditions (namely C availability) regulate S. indica’s phenotype, and its potential as plant-growth-promoting-microbe (PGPM). We grew S. indica in its free-living stage under increasing C availabilities (2–20 g L–1 of glucose or sucrose). We first characterised the effect of C availability during free-living stage growth on fungal phenotype: colonies growth and physiology (plasma membrane proton pumps, stable isotopic signatures, and potential extracellular decomposing enzymes). The effect of the C availability during the free-living stage of the PGPM was evaluated on wheat. We observed that C availability during the free-living stage regulated S. indica’s growth, ultrastructure and physiology, resulting in two distinct colony phenotypes: compact and explorer. The compact phenotype developed at low C, used peptone as the major C and N source, and displayed higher decomposing potential for C providing substrates; while the explorer phenotype developed at high C, used glucose and sucrose as major C sources and casein and yeast extract as major N sources, and displayed higher decomposing potential for N and P providing substrates. The C availability, or the C/N ratio, during the free-living stage left a legacy to the symbiosis stage, regulating S. indica’s potential to promote plant growth: wheat growth promotion by the explorer phenotype was ± 40% higher than that by the compact phenotype. Our study highlights the importance of considering microbial ecology in designing PGPM/biofertilizers. Further studies are needed to test the phenotypes under more extreme conditions, and to understand if the in vitro acquired characteristics persist under field conditions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDias T, Pimentel V, Cogo AJD, Costa R, Bertolazi AA, Miranda C, de Souza SB, Melo J, Carolino M, Varma A, Eutrópio F, Olivares FL, Ramos AC and Cruz C (2020) The Free-Living Stage Growth Conditions of the Endophytic Fungus Serendipita indica May Regulate Its Potential as Plant Growth Promoting Microbe. Front. Microbiol. 11:562238. doi: 10.3389/fmicb.2020.562238pt_PT
dc.identifier.doi10.3389/fmicb.2020.562238pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/49641
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relationFCT - PTDC/AGR-PRO/1852/2014pt_PT
dc.relationFCT - UIDB/00329/2020pt_PT
dc.relationFCT - SFRH/BPD/85419/2012pt_PT
dc.relationPostdoc grant 315/2018 EDITAL FAPES/CAPES No. 10/2018 – PROFIX 2018pt_PT
dc.relationUNIVERSAL grant 163/2019 EDITAL FAPES No. 21/2018pt_PT
dc.relationREDE INOVA CAFÉ grant 007/2020 2020-QS1DC RESOLUÇÃO FAPES No. 257/2019pt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2020.562238/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleThe Free-Living Stage Growth Conditions of the Endophytic Fungus Serendipita indica May Regulate Its Potential as Plant Growth Promoting Microbept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume11pt_PT
person.familyNameMachado Dias
person.familyNameMelo
person.familyNameCarolino
person.familyNameCruz
person.givenNameMaria Teresa
person.givenNameJuliana
person.givenNameManuela
person.givenNameCristina
person.identifierE-8816-2015
person.identifier.ciencia-idC41C-C12D-4387
person.identifier.ciencia-id881B-21C8-5F71
person.identifier.ciencia-idE416-7201-8A84
person.identifier.orcid0000-0002-5421-4763
person.identifier.orcid0000-0003-1518-5055
person.identifier.orcid0000-0001-8237-6834
person.identifier.orcid0000-0003-3100-463X
person.identifier.ridR-6003-2018
person.identifier.ridK-7876-2017
person.identifier.scopus-author-id56830642400
person.identifier.scopus-author-id55612432400
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf587ac1a-d425-4a29-827c-c9bd78edb486
relation.isAuthorOfPublicationceb63097-19b6-42d6-a8d5-b13c7cffef1a
relation.isAuthorOfPublicationca62395d-035f-43de-a845-cc2e0351cc96
relation.isAuthorOfPublicationa22a48ab-1c74-450c-a512-cdd10abd962b
relation.isAuthorOfPublication.latestForDiscoveryca62395d-035f-43de-a845-cc2e0351cc96

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