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A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies

2021-04-20Journal article Open access
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dc.contributor.authorAbade Dos Santos, F.A.
dc.contributor.authorCarvalho, Carina L.
dc.contributor.authorPeleteiro, Maria C.
dc.contributor.authorParra, Francisco
dc.contributor.authorDuarte, M. D.
dc.date.accessioned2021-06-11T14:49:18Z
dc.date.available2021-06-11T14:49:18Z
dc.date.issued2021-04-20
dc.description.abstractABSTRACT - In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly understood, since it affects reproduction, it poses an additional threat to the already fragile populations of Iberian hare, demanding prevalence investigations. In this article, we describe the first quantitative molecular method for LeHV-5 detection, using either Taqman or the EvaGreen systems. This method has excellent sensitivity and specificity, it is able to detect 2.1 copies of LeHV-5 DNA and was validated with an internal control targeting the 18S rRNA gene, allowing monitoring extraction and PCR amplification efficiencies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAbade dos Santos FA, Carvalho CL, Peleteiro MC, Parra F, Duarte MD. 2021. A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen® or Taqman® Technologies. Viruses 13(4):715. Doi: https://doi.org/10.3390/v13040715pt_PT
dc.identifier.doi10.3390/v13040715pt_PT
dc.identifier.issn1999-4915
dc.identifier.urihttp://hdl.handle.net/10400.5/21447
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAGL2017-83395-Rpt_PT
dc.relationRabbit Viral Haemorrhagic Disease control in Iberian ecosystems: dynamics of virus spread, pathophysiology and improvement of prophylactic measures.
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relation.publisherversionhttps://www.mdpi.com/1999-4915/13/4/715pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHerpesviruspt_PT
dc.subjectGammaherpesviruspt_PT
dc.subjectLeporid gammaherpesvirus 5pt_PT
dc.subjectLepus granatensispt_PT
dc.subjectIberian harept_PT
dc.subjectReproductionpt_PT
dc.subjectqPCRpt_PT
dc.titleA versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleRabbit Viral Haemorrhagic Disease control in Iberian ecosystems: dynamics of virus spread, pathophysiology and improvement of prophylactic measures.
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F137067%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleVirusespt_PT
oaire.citation.volume13(4)pt_PT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAbade dos Santos
person.familyNamePeleteiro
person.familyNameDuarte
person.givenNameFábio
person.givenNameMaria
person.givenNameMargarida
person.identifier.ciencia-idD810-0FEC-8051
person.identifier.ciencia-id6317-1F85-5E59
person.identifier.orcid0000-0002-0696-7322
person.identifier.orcid0000-0003-1848-558X
person.identifier.orcid0000-0003-1488-9659
person.identifier.ridJ-2640-2013
person.identifier.scopus-author-id6602767105
person.identifier.scopus-author-id28267670300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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