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Creating outbred and inbred populations in haplodiploids to measure adaptive responses in the laboratory

2020-07Artigo científico Acesso aberto
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dc.contributor.authorGodinho, Diogo
dc.contributor.authorCruz, Miguel A.
dc.contributor.authorCharlery de la Masselière, Maud
dc.contributor.authorTeodoro‐Paulo, Jéssica
dc.contributor.authorEira, Cátia
dc.contributor.authorDe mendonça fragata almeida, Inês
dc.contributor.authorRodrigues, Leonor R
dc.contributor.authorZélé, Flore
dc.contributor.authorMagalhaes, S
dc.date.accessioned2020-12-17T17:17:01Z
dc.date.available2020-12-17T17:17:01Z
dc.date.issued2020-07
dc.description.abstractLaboratory studies are often criticized for not being representative of processes occurring in natural populations. One reason for this is the fact that laboratory populations generally do not capture enough of the genetic variation of natural populations. This can be mitigated by mixing the genetic background of several field populations when creating laboratory populations. From these outbred populations, it is possible to generate inbred lines, thereby freezing and partitioning part of their variability, allowing each genotype to be characterized independently. Many studies addressing adaptation of organisms to their environment, such as those involving quantitative genetics or experimental evolution, rely on inbred or outbred populations, but the methodology underlying the generation of such biological resources is usually not explicitly documented. Here, we developed different procedures to circumvent common pitfalls of laboratory studies, and illustrate their application using two haplodiploid species, the spider mites Tetranychus urticae and Tetranychus evansi. First, we present a method that increases the chance of capturing high amounts of variability when creating outbred populations, by performing controlled crosses between individuals from different field-collected populations. Second, we depict the creation of inbred lines derived from such outbred populations, by performing several generations of sib-mating. Third, we outline an experimental evolution protocol that allows the maintenance of a constant population size at the beginning of each generation, thereby preventing bottlenecks and diminishing extinction risks. Finally, we discuss the advantages of these procedures and emphasize that sharing such biological resources and combining them with available genetic tools will allow consistent and comparable studies that greatly contribute to our understanding of ecological and evolutionary processes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGodinho DP, Cruz MA, Charlery de la Masselière M, et al. Creating outbred and inbred populations in haplodiploids to measure adaptive responses in the laboratory. Ecol Evol. 2020;10:7291–7305. https://doi.org/10.1002/ ece3.6454pt_PT
dc.identifier.doi10.1002/ece3.6454pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/45429
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationFCT, Grant/Award Number: PD/BD/114010/2015 and SFRH/BD/136454/2018;pt_PT
dc.relationH2020 European Research Council, Grant/Award Number: GA 725419pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleCreating outbred and inbred populations in haplodiploids to measure adaptive responses in the laboratorypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7305pt_PT
oaire.citation.issue14pt_PT
oaire.citation.startPage7291pt_PT
oaire.citation.titleEcology and Evolutionpt_PT
oaire.citation.volume10pt_PT
person.familyNameGodinho
person.familyNameLopes de Mendonça Fragata Almeida
person.familyNameRodrigues
person.familyNameZélé
person.familyNameMagalhaes
person.givenNameDiogo
person.givenNameInês Regina
person.givenNameLeonor R
person.givenNameFlore
person.givenNamesara
person.identifierResearcherID: I-9876-2014
person.identifier697927
person.identifierhttps://scholar.google.ca/citations?user=DmchGLkAAAAJ&hl=en
person.identifier.ciencia-idE91D-690E-96DC
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person.identifier.ciencia-id7319-EDE1-732A
person.identifier.ciencia-idC81C-240E-98EC
person.identifier.ciencia-id9111-5189-F926
person.identifier.orcid0000-0002-6890-5573
person.identifier.orcid0000-0001-6865-1510
person.identifier.orcid0000-0001-7871-1732
person.identifier.orcid0000-0003-2954-5488
person.identifier.orcid0000-0002-8609-7768
person.identifier.ridP-4195-2018
person.identifier.ridB-9673-2012
person.identifier.scopus-author-id24474345000
person.identifier.scopus-author-id41862732300
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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