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Synergistic and antibiofilm activity of the antimicrobial peptide P5 against carbapenem-resistant Pseudomonas aeruginosa

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dc.contributor.authorMartinez, Melina
dc.contributor.authorGonçalves, Sónia
dc.contributor.authorFelício, Mário Romão
dc.contributor.authorMaturana, Patricia
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorSemorile, Liliana
dc.contributor.authorHollmann, Axel
dc.contributor.authorMaffía, Paulo C.
dc.date.accessioned2022-05-11T16:12:02Z
dc.date.available2022-05-11T16:12:02Z
dc.date.issued2019
dc.description© 2019 Elsevier B.V. All rights reserved.pt_PT
dc.description.abstractIn the search for new antimicrobial molecules, antimicrobial peptides (AMPs) offer a viable alternative to conventional antibiotics, as they physically disrupt the bacterial membranes, leading to membrane disruption and eventually cell death. In particular, the group of linear α-helical cationic peptides has attracted increasing research and clinical interest. The AMP P5 has been previously designed as a cationic linear α-helical sequence, being its antimicrobial and hemolytic properties also evaluated. In this work, we analyzed the feasibility of using P5 against a carbapenem-resistant clinical isolate of Pseudomonas aeruginosa, one of the most common and risky pathogens in clinical practice. After antimicrobial activity confirmation in in vitro studies, synergistic activity of P5 with meropenem was evaluated, showing that P5 displayed significant synergistic activity in a time kill curve assay. The ability of P5 to permeabilize the outer membrane of P. aeruginosa can explain the obtained results. Finally, the antibiofilm activity was investigated by viability analysis (MTT assay), crystal violet and confocal imaging, with P5 displaying mild biofilm inhibition in the range of concentrations tested. Regarding biofilm disruption activity, P5 showed a higher efficacy, interfering with biofilm structure and promoting bacterial cell death. Atomic force microscope images further demonstrated the peptide potential in P. aeruginosa biofilm eradication, confirming the promising application of P5 in multi-resistant infections therapeutics.pt_PT
dc.description.sponsorshipThis work was supported by grants from Agencia Nacional de Promoción Científica y Tecnológica, Ministerio de Ciencia y Tecnología, Argentina (PICT 2016-0478 and PICT 2017-2349), CONICET (PIP-2014-11220130100383CO), Universidad Nacional de Quilmes (Programa Microbiología Molecular Básica y Aplicada) and Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal). LS is a member of the Research Career of CIC-BA. AH and PCM are members of the Carrera del Investigador Cientıfico y Tecnologico (CONICET, Argentina). MM. and PM acknowledge fellowships from CONICET.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiochim Biophys Acta Biomembr. 2019 Jul 1;1861(7):1329-1337pt_PT
dc.identifier.doi10.1016/j.bbamem.2019.05.008pt_PT
dc.identifier.eissn1879-2642
dc.identifier.issn0005-2736
dc.identifier.urihttp://hdl.handle.net/10451/52904
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/biochimica-et-biophysica-acta-bba-biomembranespt_PT
dc.subjectAntibiofilm peptidept_PT
dc.subjectAntimicrobial peptidept_PT
dc.subjectBiofilmpt_PT
dc.subjectMeropenempt_PT
dc.subjectPseudomonas aeruginosa;pt_PT
dc.subjectSynergistic activitypt_PT
dc.titleSynergistic and antibiofilm activity of the antimicrobial peptide P5 against carbapenem-resistant Pseudomonas aeruginosapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1337pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1329pt_PT
oaire.citation.titleBiochimica et Biophysica Acta (BBA) - Biomembranespt_PT
oaire.citation.volume1861pt_PT
person.familyNameRomão Felício
person.familyNameSantos
person.givenNameMário
person.givenNameNuno
person.identifier384205
person.identifier.ciencia-idCD13-5E3A-A3C5
person.identifier.orcid0000-0002-9398-2140
person.identifier.orcid0000-0002-0580-0475
person.identifier.ridJ-8517-2014
person.identifier.ridN-7248-2013
person.identifier.scopus-author-id57144367400
person.identifier.scopus-author-id55940818300
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8d7bbafe-ae5e-418e-8224-6af22991a3e7
relation.isAuthorOfPublication4656d912-5a2d-4c80-8921-2bcba3aa441a
relation.isAuthorOfPublication.latestForDiscovery4656d912-5a2d-4c80-8921-2bcba3aa441a

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