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Marine heat waves alter gene expression of key enzymes of membrane and storage lipids metabolism in Phaeodactylum tricornutum

2020Journal article Restricted access
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dc.contributor.authorFeijão, Eduardo
dc.contributor.authorFranzitta, Marco
dc.contributor.authorCabrita, Maria Teresa
dc.contributor.authorCaçador, Isabel
dc.contributor.authorDuarte, Bernardo
dc.contributor.authorGameiro, Carla
dc.contributor.authorMatos, Ana Rita
dc.date.accessioned2021-05-10T13:14:16Z
dc.date.available2021-05-10T13:14:16Z
dc.date.issued2020
dc.description.abstractAcross the globe, heat waves are getting more intense and frequent. Diatoms are a major group of microalgae at the base of the marine food webs and an important source of long chain polyunsaturated fatty acids that are transferred through the food web. The present study investigates the possible impacts of temperature increase on lipid classes and expression of genes encoding enzymes related to lipid metabolism in Phaeodactylum tricornutum. The heat wave exposure caused an increase in the relative amounts of plastidial lipids such as the glycolipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulphoquinovosyldiacylglycerol (SQDG) in parallel with a decrease in the neutral lipid fraction, which includes triacylglycerols. In agreement, gene expression analyses revealed an up-regulation of a gene encoding one MGDG synthase and down-regulation of a diacylglycerol acyltransferase (DGAT), a key enzyme in triacylglycerol synthesis. Our results show that heat waves not only negatively impact the abundance of unsaturated fatty acids such as eicosapentaenoic acid (20:5n-3, EPA) and hexadecatrienoic acid (16:3n-4) as observed by the decrease in their relative abundance in MGDG and neutral lipids, respectively, but also induce changes in the relative amounts of the diverse membrane lipids as well as the proportion of membrane/storage lipids. The expression study of key genes indicates that some of the aforementioned alterations are regulated at the transcription level whereas others appear to be post-transcriptional. The changes observed in plastidial lipids are related to negative impacts on the photosynthesis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFeijão, E., Franzitta, M., Cabrita, M. T., Caçador, I., Duarte, B., Gameiro, C., Matos, A. R. (2020). Marine heat waves alter gene expression of key enzymes of membrane and storage lipids metabolism in Phaeodactylum tricornutum. Plant Physiology and Biochemistry, 156, 357-368. https://doi.org/10.1016/j.plaphy.2020.09.022pt_PT
dc.identifier.doi10.1016/j.plaphy.2020.09.022pt_PT
dc.identifier.issn0981-9428
dc.identifier.urihttp://hdl.handle.net/10451/47719
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S098194282030471Xpt_PT
dc.subjectDiatomspt_PT
dc.subjectGene Expressionpt_PT
dc.subjectLipid Metabolismpt_PT
dc.subjectLipidspt_PT
dc.subjectPlastidspt_PT
dc.subjectHot Temperaturept_PT
dc.titleMarine heat waves alter gene expression of key enzymes of membrane and storage lipids metabolism in Phaeodactylum tricornutumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage368pt_PT
oaire.citation.startPage357pt_PT
oaire.citation.titlePlant Physiology and Biochemistrypt_PT
oaire.citation.volume156pt_PT
person.familyNameCabrita
person.givenNameMaria Teresa
person.identifier349469
person.identifier.ciencia-id3E17-0125-F909
person.identifier.orcid0000-0002-4323-4945
person.identifier.ridB-5746-2014
person.identifier.scopus-author-id6602224932
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationcac05d5d-1ba9-4d1a-a052-d8bde9ecfadc
relation.isAuthorOfPublication.latestForDiscoverycac05d5d-1ba9-4d1a-a052-d8bde9ecfadc

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