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Blood collection from the external jugular vein of Oryctolagus cuniculus algirus sedated with midazolam: live sampling of a subspecies at risk

2019-12Journal article Open access
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dc.contributor.authorAbade dos Santos, Fabio A.
dc.contributor.authorCarvalho, Carina L.
dc.contributor.authorPeleteiro, M. Conceição
dc.contributor.authorGabriel, Sofia Isabel
dc.contributor.authorPatrício, Rui
dc.contributor.authorCarvalho, João
dc.contributor.authorCunha, Mónica V.
dc.contributor.authorDuarte, Margarida D.
dc.date.accessioned2021-01-31T14:02:20Z
dc.date.available2021-01-31T14:02:20Z
dc.date.issued2019-12
dc.description.abstractIn the last decades, the European wild rabbit, particularly the Oryctolagus cuniculus algirus, a keystone species in the Iberian Peninsula ecosystems, declined severely, raising concerns from the wildlife authorities. The hunting calendar in Portugal limits sampling collection to a narrow window of few months annually. Nevertheless, governmental wildlife protection laws allow live rabbit sampling for population and sanitary evaluations. The aim of this study is to adjust blood collection protocols from the external jugular vein (EJV) described for domestic rabbits to the wild rabbit. Collection of peripheral blood is problematic in the wild rabbit given its small body size and the reduced calibre of vessels but mostly its nervous disposition and fragility. We describe in detail a procedure for EJV blood collection in 30 wild rabbits after sedation with midazolam. Emphasis is given to protocol adjustments for wild rabbit. Heart rate, respiratory rate and body temperature were assessed before sedation, after sedation but before collection and after blood collection. Sedation onset took on mean (SD) 8 ± 2 min. The technique allowed the collection at least 1 ml of blood, a satisfactory volume for routine laboratory testing. The differences observed in heart and respiratory rates before and after blood collection were not statistically sig-nificant, indicating that no cardiorespiratory interference occurred due to venepuncture. Recovery from sedation took on mean (SD) 17 ± 2 min. All animals were set free during the first hour after blood collection. This work aims to demonstrate that blood collection under sedation is a safe and feasible procedure in wild rabbits when practiced by experienced veteri-narians. At no time, whatsoever, was the physiological homeostasis at risk and no injuries were inflicted on the animals. To our knowledge this report constitutes the first guided description of blood collection from the EJV in sedated O. c. algirusand the first collection of physiological parameters measured under different conditions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFabio A. Abade dos Santos, Carina L. Carvalho, M. Conceição Peleteiro, Sofia Isabel Gabriel, Rui Patrício, João Carvalho, Mónica V. Cunha, Margarida D. Duarte "Blood collection from the external jugular vein of Oryctolagus cuniculus algirus sedated with midazolam: live sampling of a subspecies at risk," Wildlife Biology, 1: 1-10 (2019)pt_PT
dc.identifier.doi10.2981/wlb.00588pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/46086
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBioOnept_PT
dc.relationFCT UID/CVT/00276/2013pt_PT
dc.relationFCT SFRH/BD/137067/2018pt_PT
dc.relationFCT UID/AMB/50017/2019pt_PT
dc.relationFundo Florestal Permanente (Government of Portugal) in the scope of the Action Plan for the Control of Rabbit Viral Haemorrhagic Disease (Dispatch no. 4757/2017 of 31 May)pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBlood collectionpt_PT
dc.subjectExternal jugular veinpt_PT
dc.subjectOryctolagus cuniculuspt_PT
dc.subjectSampling live animalspt_PT
dc.subjectSanitary surveillancept_PT
dc.subjectVenepuncturept_PT
dc.subjectWild rabbitpt_PT
dc.titleBlood collection from the external jugular vein of Oryctolagus cuniculus algirus sedated with midazolam: live sampling of a subspecies at riskpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titleWildlife Biologypt_PT
oaire.citation.volume2019pt_PT
person.familyNameCunha
person.givenNameMonica V.
person.identifier348477
person.identifier.ciencia-id5616-E715-2F31
person.identifier.orcid0000-0003-0401-0276
person.identifier.ridD-5544-2011
person.identifier.scopus-author-id7102711216
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa1b365bb-7421-4bf5-9cde-62b74c170e69
relation.isAuthorOfPublication.latestForDiscoverya1b365bb-7421-4bf5-9cde-62b74c170e69

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