Logo do repositório
 
Publicação

Genome analysis of Legionella pneumophila ST23 from various countries reveals highly similar strains

2022Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorRicci, Maria Luisa
dc.contributor.authorFillo, Silvia
dc.contributor.authorCiammaruconi, Andrea
dc.contributor.authorLista, Florigio
dc.contributor.authorGinevra, Christophe
dc.contributor.authorJarraud, Sophie
dc.contributor.authorGirolamo, Antonietta
dc.contributor.authorBarbanti, Fabrizio
dc.contributor.authorRota, Maria Cristina
dc.contributor.authorLindsay, Diane
dc.contributor.authorGorzynski, Jamie
dc.contributor.authorUldum, Søren A
dc.contributor.authorBaig, Sharmin
dc.contributor.authorFoti, Marina
dc.contributor.authorPetralito, Giancarlo
dc.contributor.authorTorri, Stefania
dc.contributor.authorFaccini, Marino
dc.contributor.authorBonini, Maira
dc.contributor.authorGentili, Gabriella
dc.contributor.authorSenatore, Sabrina
dc.contributor.authorLamberti, Anna
dc.contributor.authorCarrico, Joao Andre
dc.contributor.authorScaturro, Maria
dc.date.accessioned2022-03-25T16:41:34Z
dc.date.available2022-03-25T16:41:34Z
dc.date.issued2022
dc.description© 2022 Ricci et al. This article is available under a CreativeCommons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractLegionella pneumophila serogroup 1 (Lp1) sequence type (ST) 23 is one of the most commonly detected STs in Italy where it currently causes all investigated outbreaks. ST23 has caused both epidemic and sporadic cases between 1995 and 2018 and was analysed at genomic level and compared with ST23 isolated in other countries to determine possible similarities and differences. A core genome multi-locus sequence typing (cgMLST), based on a previously described set of 1,521 core genes, and single-nucleotide polymorphisms (SNPs) approaches were applied to an ST23 collection including genomes from Italy, France, Denmark and Scotland. DNAs were automatically extracted, libraries prepared using NextEra library kit and MiSeq sequencing performed. Overall, 63 among clinical and environmental Italian Lp1 isolates and a further seven and 11 ST23 from Denmark and Scotland, respectively, were sequenced, and pangenome analysed. Both cgMLST and SNPs analyses showed very few loci and SNP variations in ST23 genomes. All the ST23 causing outbreaks and sporadic cases in Italy and elsewhere, were phylogenetically related independent of year, town or country of isolation. Distances among the ST23s were further shortened when SNPs due to horizontal gene transfers were removed. The Lp1 ST23 isolated in Italy have kept their monophyletic origin, but they are phylogenetically close also to ST23 from other countries. The ST23 are quite widespread in Italy, and a thorough epidemiological investigation is compelled to determine sources of infection when this ST is identified in both LD sporadic cases and outbreaks.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLife Sci Alliance. 2022 Mar 2;5(6):e202101117pt_PT
dc.identifier.doi10.26508/lsa.202101117pt_PT
dc.identifier.eissn2575-1077
dc.identifier.urihttp://hdl.handle.net/10451/51991
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherLife Science Alliance LLC.pt_PT
dc.relation.publisherversionhttps://www.life-science-alliance.org/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleGenome analysis of Legionella pneumophila ST23 from various countries reveals highly similar strainspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6pt_PT
oaire.citation.titleLife Science Alliancept_PT
oaire.citation.volume5pt_PT
person.familyNameCarrico
person.givenNameJoao
person.identifier391563
person.identifier.orcid0000-0002-5274-2722
person.identifier.ridA-7367-2008
person.identifier.scopus-author-id8266333200
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione4e2a0de-797a-4935-8b91-134efe7b784e
relation.isAuthorOfPublication.latestForDiscoverye4e2a0de-797a-4935-8b91-134efe7b784e

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Analysis_Legionella.pdf
Tamanho:
2 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

FM - Artigos em Revistas Internacionais