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A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
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Orientador(es)
Resumo(s)
The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation. We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria. Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells. Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former. This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.
Descrição
© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Palavras-chave
Nioorthogonal chemistry Carbohydrates GLUT1 transporters Inverse electron-demand Diels-Alder Malaria
Contexto Educativo
Citação
Chembiochem. 2020 Sep 14;21(18):2696-2700
Editora
John Wiley & Sons, Inc.