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The unique biosynthetic route from Lupinus Beta-conglutin gene to blad
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Orientador(es)
Resumo(s)
Background: During seed germination, b-conglutin undergoes a major cycle of limited proteolysis in which many of its
constituent subunits are processed into a 20 kDa polypeptide termed blad. Blad is the main component of a glycooligomer,
accumulating exclusively in the cotyledons of Lupinus species, between days 4 and 12 after the onset of germination.
Principal Findings: The sequence of the gene encoding b-conglutin precursor (1791 nucleotides) is reported. This gene,
which shares 44 to 57% similarity and 20 to 37% identity with other vicilin-like protein genes, includes several features in
common with these globulins, but also specific hallmarks. Most notable is the presence of an ubiquitin interacting motif
(UIM), which possibly links the unique catabolic route of b-conglutin to the ubiquitin/proteasome proteolytic pathway.
Significance: Blad forms through a unique route from and is a stable intermediary product of its precursor, b-conglutin, the
major Lupinus seed storage protein. It is composed of 173 amino acid residues, is encoded by an intron-containing, internal
fragment of the gene that codes for b-conglutin precursor (nucleotides 394 to 913) and exhibits an isoelectric point of 9.6
and a molecular mass of 20,404.85 Da. Consistent with its role as a storage protein, blad contains an extremely high
proportion of the nitrogen-rich amino acids
Descrição
Palavras-chave
Lupinus blad
Contexto Educativo
Citação
Monteiro S, Freitas R, Rajasekhar BT, Teixeira AR, Ferreira RB (2010) The Unique Biosynthetic Route from Lupinus b-Conglutin Gene to Blad. PLoS ONE 5(1): e8542
Editora
Plos ONE