A carregar...
Publicação
Progressive long-term synaptic depression at cortical inputs into the amygdala
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 4.1 MB | Adobe PDF |
Orientador(es)
Resumo(s)
The convergence of conditioned and unconditioned stimuli (CS and US) into the lateral amygdala (LA) serves as a substrate for an adequate fear response in vivo. This well-known Pavlovian paradigm modulates the synaptic plasticity of neurons, as can be proved by the long-term potentiation (LTP) phenomenon in vitro. Although there is an increasing body of evidence for the existence of LTP in the amygdala, only a few studies were able to show a reliable long-term depression (LTD) of excitation in this structure. We have used coronal brain slices and conducted patch-clamp recordings in pyramidal neurons of the lateral amygdala (LA). After obtaining a stable baseline excitatory postsynaptic current (EPSC) response at a holding potential of -70 mV, we employed a paired-pulse paradigm at 1 Hz at the same membrane potential and could observe a reliable LTD. The different durations of stimulation (ranging between 1.5-24 min) were tested first in the same neuron, but the intensity was kept constant. The latter paradigm resulted in a step-wise LTD with a gradually increasing magnitude under these conditions.
Descrição
© 2024 International Brain Research Organization (IBRO). Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies
Palavras-chave
Action potential Fear circuit Long-term synaptic plasticity Patch-clamp Spike
Contexto Educativo
Citação
Neuroscience. 2024 Sep 25:556:52-65
Editora
Elsevier